OCT1 Colorimetric Cell-Based ELISA Kit

Product Name

OCT1 Colorimetric Cell-Based ELISA Kit

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SKU/Catalog Number

EKC1003

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The OCT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT1.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

OCT1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1003)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1003 is reactive to POU2F1 in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1003 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	1 Plates	-
10x TBS	24 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	50 ml (15x)	Clear
100x Anti-OCT1 Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	6ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	6ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	6ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For POU2F1 (Source: Uniprot.Org, NCBI)

Alternative Names

NF-A1;OCT1;OTF-1;Octamer-binding transcription factor 1;PO21;PO2F1;POU domain, class 2;transcription factor 1;POU2F1 POU2F1 OCT1, OTF1, oct-1B POU class 2 homeobox 1 POU domain, class 2, transcription factor 1|NF-A1|OTF-1|Octamer-binding transcription factor-1|oct-1|octamer-binding protein 1|octamer-binding transcription factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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