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Product Info Summary
| SKU: | A09480 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NUPL2/NUP42 Antibody Picoband®
SKU/Catalog Number
A09480
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUPL2/NUP42 Antibody Picoband® catalog # A09480. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUPL2/NUP42 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09480)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUPL2/NUP42 recombinant protein (Position: Q52-V423).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09480 is reactive to NUP42 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
44.872kDa
Background of NUP42
NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09480 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human THP-1 whole cell, human K562 whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NUPL2/NUP42 using anti-NUPL2/NUP42 antibody (A09480).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUPL2/NUP42 antigen affinity purified polyclonal antibody (Catalog # A09480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUPL2/NUP42 at approximately 36 kDa. The expected band size for NUPL2/NUP42 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NUP42 (Source: Uniprot.org, NCBI)
Gene Name
NUP42
Full Name
Nucleoporin NUP42
Weight
44.872kDa
Alternative Names
Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP; NUP42 CG1, NLP-1, NLP_1, NUPL2, hCG1 nucleoporin 42 nucleoporin NUP42|H_RG271G13.9|NUP42 homolog|nucleoporin hCG1|nucleoporin like 2|nucleoporin-like protein 1|nucleoporin-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUP42, check out the NUP42 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUP42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUPL2/NUP42 Antibody Picoband® (A09480)
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