Anti-Nucleostemin/GNL3 Antibody Picoband®

Nucleostemin antibody

Boster Bio Anti-Nucleostemin/GNL3 Antibody Picoband® catalog # A03283-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03283-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Nucleostemin/GNL3 Antibody Picoband®

View all Nucleostemin Antibodies

SKU/Catalog Number

A03283-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nucleostemin/GNL3 Antibody Picoband® catalog # A03283-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Nucleostemin/GNL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03283-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Nucleostemin/GNL3 recombinant protein (Position: R45-V549).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03283-3 is reactive to GNL3 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

65 kDa

Calculated molecular weight

27683 MW

Background of Nucleostemin

Guanine nucleotide-binding protein-like 3, also known as nucleostemin, is a protein that in humans is encoded by the GNL3 gene. The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03283-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human Hela whole cell, human K562 whole cell, human PC-3 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: human appendiceal adenocarcinoma tissue, human breast cancer tissue, human colonic adenocarcinoma tissue, human gall bladder adenosquamous carcinoma tissue
ICC/IF: HEP3B cell
FCM: Hela cell

Validation Images & Assay Conditions

Gene/Protein Information For GNL3 (Source: Uniprot.org, NCBI)

Gene Name

GNL3

Full Name

Guanine nucleotide-binding protein-like 3

Weight

27683 MW

Superfamily

TRAFAC class YlqF/YawG GTPase family

Alternative Names

CD48 antigen;B-lymphocyte activation marker BLAST-1;BCM1 surface antigen;Leukocyte antigen MEM-102;SLAM family member 2;SLAMF2;Signaling lymphocytic activation molecule 2;TCT.1;CD48;CD48;BCM1, BLAST1; GNL3 C77032, E2IG3, NNP47, NS G protein nucleolar 3 guanine nucleotide-binding protein-like 3|E2-induced gene 3 protein|estradiol-induced nucleotide binding protein|guanine nucleotide binding protein-like 3 (nucleolar)|novel nucleolar protein 47|nucleolar GTP-binding protein 3|nucleostemin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GNL3, check out the GNL3 Infographic

GNL3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GNL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03283-3

100μg

$370
A03283-3-10ug

10μg sample (liquid)

$99
A03283-3-Biotin

100 μg Biotin conjugated

$570
A03283-3-Cy3

100 μg Cy3 conjugated

$570
A03283-3-Dylight488

100 μg Dylight488 conjugated

$570
A03283-3-Dylight550

100 μg Dylight550 conjugated

$570
A03283-3-Dylight594

100 μg Dylight594 conjugated

$570
A03283-3-FITC

100 μg FITC conjugated

$570
A03283-3-HRP

100 μg HRP conjugated

$570
A03283-3-APC

100 μg APC conjugated

$670
A03283-3-PE

100 μg PE conjugated

$670
A03283-3-iFluor647

100 μg iFluor647 conjugated

$670
A03283-3-carrier-free

Carrier Free

$370
Rainbow Button View conjugates

Free Secondary Antibody

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A03283-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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