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Product Info Summary
| SKU: | M00228-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nucleolin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00228-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Nucleolin Rabbit Monoclonal Antibody catalog # M00228-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nucleolin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00228-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30N86
Isotype
IgG
Immunogen
A synthesized peptide derived from human Nucleolin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00228-3 is reactive to NCL in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
36053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00228-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of Nucleolin expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NCL (Source: Uniprot.org, NCBI)
Gene Name
NCL
Full Name
Nucleolin
Weight
36053 MW
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; NCL C23, Nsr1 nucleolin nucleolin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCL, check out the NCL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nucleolin Rabbit Monoclonal Antibody (M00228-3)
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No publications found for M00228-3
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