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Product Info Summary
| SKU: | PB9876 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Nucleobindin 2/NUCB2 Antibody Picoband®
View all Nesfatin-1/Nucleobindin-2 Antibodies
SKU/Catalog Number
PB9876
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Nucleobindin 2/NUCB2 Antibody Picoband® catalog # PB9876. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nucleobindin 2/NUCB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9876)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9876 is reactive to Nucb2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50304 MW
Background of Nesfatin-1/Nucleobindin-2
Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9876 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: HEPA whole cell
IHC: mouse gaster tissue, rat gaster tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HEPA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleobindin 2 antigen affinity purified polyclonal antibody (Catalog # PB9876) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleobindin 2 at approximately 55 kDa. The expected band size for Nucleobindin 2 is at 50 kDa.
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Figure 2. IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876).
Nucleobindin 2 was detected in a paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876).
Nucleobindin 2 was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nucb2 (Source: Uniprot.org, NCBI)
Gene Name
Nucb2
Full Name
Nucleobindin-2
Weight
50304 MW
Superfamily
nucleobindin family
Alternative Names
Nucleobindin-2;DNA-binding protein NEFA;Prepronesfatin;Nesfatin-1;Nucb2;Nefa; NUCB2 HEL-S-109, NEFA nucleobindin 2 nucleobindin-2|DNA-binding protein NEFA|epididymis secretory protein Li 109|gastric cancer Zg4|nesfatin 1|novel DNA binding/EF-hand/leucine zipper protein|nucleobinding 2|prepronesfatin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nucb2, check out the Nucb2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nucb2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nucleobindin 2/NUCB2 Antibody Picoband® (PB9876)
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Customer Q&As
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7 Customer Q&As for Anti-Nucleobindin 2/NUCB2 Antibody Picoband®
Question
I have a question about product PB9876, anti-Nucleobindin 2/NUCB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Roberts
Verified customer
Asked: 2020-04-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9876 anti-Nucleobindin 2/NUCB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-30
Question
We are currently using anti-Nucleobindin 2/NUCB2 antibody PB9876 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Nucleobindin 2/NUCB2 antibody (PB9876) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Nucleobindin 2/NUCB2 antibody PB9876. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
Would anti-Nucleobindin 2/NUCB2 antibody PB9876 work for ELISA with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
According to the expression profile of cervix carcinoma, NUCB2 is highly expressed in cervix carcinoma. So, it is likely that anti-Nucleobindin 2/NUCB2 antibody PB9876 will work for ELISA with cervix carcinoma.
Boster Scientific Support
Answered: 2019-08-14
Question
Do you have a BSA free version of anti-Nucleobindin 2/NUCB2 antibody PB9876 available?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Nucleobindin 2/NUCB2 antibody PB9876 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-07
Question
We are interested in to test anti-Nucleobindin 2/NUCB2 antibody PB9876 on rat cervix carcinoma for research purposes, then I may be interested in using anti-Nucleobindin 2/NUCB2 antibody PB9876 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The products we sell, including anti-Nucleobindin 2/NUCB2 antibody PB9876, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-17
Question
Would anti-Nucleobindin 2/NUCB2 antibody PB9876 work on feline WB with saliva-secreting gland?
W. Rodriguez
Verified customer
Asked: 2014-06-05
Answer
Our lab technicians have not validated anti-Nucleobindin 2/NUCB2 antibody PB9876 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Nucleobindin 2/NUCB2 antibody PB9876 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline saliva-secreting gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-05
