Click image to see more details
Product Info Summary
| SKU: | A00051-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-StAR Antibody Picoband®
SKU/Catalog Number
A00051-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-StAR Antibody Picoband® catalog # A00051-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-StAR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00051-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human StAR, which shares 85% and 87.5% amino acid (aa) sequence identity with mouse and rat StAR, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00051-1 is reactive to STAR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
25283 MW
Background of STAR
The steroidogenic acute regulatory protein, commonly referred to as StAR (STARD1), is a transport protein. This protein plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. It permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00051-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of StAR using anti-StAR antibody (A00051-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
Protein Target Info & Infographic
Gene/Protein Information For STAR (Source: Uniprot.org, NCBI)
Gene Name
STAR
Full Name
Steroidogenic acute regulatory protein, mitochondrial
Weight
25283 MW
Alternative Names
Steroidogenic acute regulatory protein, mitochondrial; StAR; START domain-containing protein 1; StARD1; STAR; STARD1 STAR STARD1 steroidogenic acute regulatory protein steroidogenic acute regulatory protein, mitochondrial|START domain containing 1|START domain-containing protein 1|StAR related lipid transfer (START) domain containing 1|cholesterol trafficker|mitochondrial steroid acute regulatory protein|steroid acute regulatory protein|steroidogenic acute regulator|testis secretory sperm-binding protein Li 241mP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAR, check out the STAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-StAR Antibody Picoband® (A00051-1)
Hello CJ!
A00051-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
4‐Methylcatechol inhibits cell growth and testosterone production in TM3 Leydig cells by reducing mitochondrial activity
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats
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Customer Q&As
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1 Customer Q&As for Anti-StAR Antibody Picoband®
Question
We are currently using anti-StAR antibody A00051-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
The anti-StAR antibody (A00051-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-16
