Nucleobindin-2 Antibodies

Nucleobindin-2 (NUCB2)

Calcium-binding protein. May have a role in calcium homeostasis.

.

Gene Information

Human
Gene Name:	NUCB2
Uniprot:	P80303
Entrez:	4925

Family

Belongs to:
nucleobindin family

Alternative Names

DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2

Molecular Weight

Mass (kDA):
50.223 kDA

Genomic Context

Human
Location:	11p15.1
Sequence:	11; NC_000011.10 (17260337..17349980)

Tissue Specificity

Predominantly expressed in spleen, testis and normal stomach.

Subcellular Localizations

Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted. Endoplasmic reticulum. Nucleus envelope. Golgi retention is mediated by its N-terminal region.; [Nesfatin-1]: Secreted.

Diseases

• Obesity
• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Nervousness
• Disease Of Adrenal Medulla
• Pituitary Diseases
• Stress, Psychological
• Malignant Neoplasms
• Stomach Neoplasms
• Neoplasms
• Anxiety Disorders
• Mental Disorders

• Diabetes Mellitus, Insulin-dependent
• Insulin Resistance
• Metabolic Diseases
• Malignant Neoplasm Of Stomach
• Neoplasm Metastasis
• Metastatic Malignant Neoplasm To The Lymph Node
• Malignant Neoplasm Of Breast

Interacting Proteins

• GADD45A

Pathways

• Secretion
• Energy Homeostasis
• Insulin Secretion
• Localization
• Feeding Behavior
• Pathogenesis
• Transport
• Immune Response
• Inflammatory Response
• Glucose Homeostasis
• Innate Immune Response
• Testosterone Secretion
• Regulation Of Feeding Behavior

• Exocytosis
• Glucagon Secretion
• Follicle-stimulating Hormone Secretion
• Hormone Secretion
• Cell Proliferation
• Blood Vessel Development
• Induction Of Apoptosis

PTMs

• Phosphorylation

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NUCB2

Boster Bio - Best Uses Of The NUCB2 Marker

Boster Bio is a well-known company that makes Anti-Human Biotinylated Antibodies. What exactly is the NUCB2 marker? What can it do for you? Both are covered in this article. Continue reading to learn more about the biotinylated antibody. Learn about its benefits for your experiments. It's not only for biologists. It's available to researchers from all over the world So there's no reason not to get your hands on it!

Boster Bio

When selecting a biological marker for your research, there are a variety of factors that you must consider. These factors can have an impact on the outcome of your study. For instance, the target species should be immunogenic to Boster Bio antibodies, so your study should be positive for NUCB2 in the event that your specimens match. Boster Bio antibodies are also the reagents that have been tested on multiple platforms.

EK1138-CAP Anti-Human NUCB2

Boster Bio's EK1138-CAP Anti-Humen NUCB2 Marker is a highly specific and sensitive antibody to detect of human NUCB2. This is a multiplatform antibody that has been validated to be used in a variety of tests. It has been tested in IHC as well as ELISA and WB. It reacts with Rats as well as Mouse.

EK1138 DA Anti-Human NUCB2 Biotinylated Antibody

Boster Bio EK1138 DA Human NUCB2 biotinylated antibody is a new product in their Picoband (tm). This antibody is made to recognize Human NUCB2. It has been tested in IHC and WB as well as ELISA. It is available in 100ul Liquid.

The process of developing begins with the titration ELISA tests. After each test-bleed the spleen cells are separated. Hybridoma fusion can be performed using the animals that show the highest response. A ELISA test is used to determine positive supernatants. The selected antibodies go through further application testing. The n-glycan protein is an unusual protein, however an antibody that recognizes it is very specific.

Boster's Nesfatin-1 ELISA Kit utilizes the traditional sandwich ELISA Technology. A monoclonal antibody to human that is specific for Nesfatin-1, is first precoated on plates with 96 wells. Next, the goat biotinylated monoclonal antibody is added. The unbound conjugates were eliminated using TBS or PBS buffer.

Secondary antibodies are usually utilized to amplify the signal produced by the primary antibody. To prevent non-specific binding and false positives, secondary antibodies need to be made from a different species to the primary antibody or specimen. The amount of dilutions can be increased based on the specificity required. Incubation should take one hour at 37degC or up to two hours at room temperature. If you preferto do it overnight, then overnight incubation may be possible.

Human NUCB2 is an extremely versatile cytokine for immunoregulation. Its variants can increase the risk of skin squamous-cell cancers following renal transplantation as well as sudden infant death. The interleukin-10 locus may be associated with the heritability of and occurrence of psoriasis.

To avoid background staining using the Boster Bio NUCB2 antibody EK1138-DA Human NUCB2, ensure you utilize a dilution buffer. For larger samples, use a multichannel pipette. When you share your results with your colleagues Don't forget to award Boster Bio EK1138-DA Anti-Human NUCB2 Biotinylated antibody some product credit!