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Product Info Summary
| SKU: | A14044 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NSA1 WDR74 Antibody
SKU/Catalog Number
A14044
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NSA1 WDR74 Antibody (Catalog # A14044). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NSA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NSA1 WDR74 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14044)
Host
Rabbit
Contents
NSA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NSA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NSA1. The immunogen is located within amino acids 320 - 370 of NSA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of TMEM135 are known to exist; this antibody will detect both isoforms.
Reactive Species
A14044 is reactive to WDR74 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
42441 MW
Background of WDR74
The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14044 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NSA1 in mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NSA1 in rat liver tissue with NSA1 antibody at 5 μg/mL.
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Immunofluorescence of NSA1 in rat liver tissue with NSA1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For WDR74 (Source: Uniprot.org, NCBI)
Gene Name
WDR74
Full Name
WD repeat-containing protein 74
Weight
42441 MW
Alternative Names
NSA1, WD repeat-containing protein 74, NOP seven-associated protein 1 WDR74 Nsa1 WD repeat domain 74 WD repeat-containing protein 74|NOP seven-associated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WDR74, check out the WDR74 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NSA1 WDR74 Antibody (A14044)
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