Product Info Summary
SKU: | PA1820 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Kallikrein 9/KLK9 Antibody Picoband®
View all Kallikrein 9 Antibodies
SKU/Catalog Number
PA1820
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 9/KLK9 Antibody catalog # PA1820. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 9/KLK9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1820)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1820 is reactive to KLK9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
27513 MW
Background of Kallikrein 9
KLK9 (kallikrein-related peptidase 9) also known as kallikrein 9 or KLK-L3, belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. The KLK9 gene contains 5 coding exons and is mapped to 19q13.41. The KLK9 gene is regulated by steroid hormones in a human breast cancer cell line. Yousef et al. (2001) performed a quantitative analysis of KLK9 expression in ovarian cancer. The results indicated thatKLK9 is under steroid hormone regulation in ovarian and breast cancer cell lines. KLK9 is a potential independent favorable prognostic marker for early-stage, low-grade, optimally debulked ovarian cancer patients.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1820 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Cell, A431 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-Kallikrein 9 antibody, PA1820, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: A431 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For KLK9 (Source: Uniprot.org, NCBI)
Gene Name
KLK9
Full Name
Kallikrein-9
Weight
27513 MW
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-9;3.4.21.-;Kallikrein-like protein 3;KLK-L3;KLK9; KLK9 KLK-L3, KLKL3 kallikrein related peptidase 9 kallikrein-9|kallikrein-like protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK9, check out the KLK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 9/KLK9 Antibody Picoband® (PA1820)
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Customer Q&As
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6 Customer Q&As for Anti-Kallikrein 9/KLK9 Antibody Picoband®
Question
We need to test anti-Kallikrein 9/KLK9 antibody PA1820 on human ectocervix for research purposes, then I may be interested in using anti-Kallikrein 9/KLK9 antibody PA1820 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The products we sell, including anti-Kallikrein 9/KLK9 antibody PA1820, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-25
Question
Is this PA1820 anti-Kallikrein 9/KLK9 antibody reactive to the isotypes of KLK9?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The immunogen of PA1820 anti-Kallikrein 9/KLK9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 9(236-250aa SVCHYLDWIQEIMEN). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-27
Question
My question regarding product PA1820, anti-Kallikrein 9/KLK9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Wu
Verified customer
Asked: 2019-07-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1820 anti-Kallikrein 9/KLK9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-09
Question
Is a blocking peptide available for product anti-Kallikrein 9/KLK9 antibody (PA1820)?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
We do provide the blocking peptide for product anti-Kallikrein 9/KLK9 antibody (PA1820). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-09
Question
We are currently using anti-Kallikrein 9/KLK9 antibody PA1820 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
N. Lewis
Verified customer
Asked: 2017-06-05
Answer
The anti-Kallikrein 9/KLK9 antibody (PA1820) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-05
Question
Would PA1820 anti-Kallikrein 9/KLK9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Brown
Verified customer
Asked: 2016-07-20
Answer
As indicated on the product datasheet, PA1820 anti-Kallikrein 9/KLK9 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-20