Anti-Neuropilin-2 NRP2 Antibody

Product Name

Anti-Neuropilin-2 NRP2 Antibody

View all Neuropilin-2 Antibodies

SKU/Catalog Number

PA1771

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neuropilin-2 NRP2 Antibody catalog # PA1771. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neuropilin-2 NRP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1771)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NRP2, identical to the related mouse sequence and different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1771 is reactive to NRP2 in Human, Mouse, Rat

Applications

PA1771 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

105 kDa

Calculated molecular weight

104859 MW

Background of Neuropilin-2

NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped on 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. Sema3b can act to repulse axons expressing neuropilin-2 and can antagonize the repulsive action of Sema3a on axonal growth cones expressing neuropilin-1. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in Npn2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Npn2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-NRP2 antibody, PA1771, Western blotting
All lanes: Anti NRP2 (PA1771) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Predicted bind size: 105KD
Observed bind size: 105KD

Gene/Protein Information For NRP2 (Source: Uniprot.org, NCBI)

Alternative Names

MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17) NRP2 NP2, NPN2, PRO2714, VEGF165R2 neuropilin 2 neuropilin-2|neuropilin-2a(17)|neuropilin-2a(22)|neuropilin-2b(0)|receptor for VEGF165 and semaphorins class3|vascular endothelial cell growth factor 165 receptor 2

For more info on NRP2, check out the NRP2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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