Product Info Summary
SKU: | PA1771 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Neuropilin-2 NRP2 Antibody Picoband®
View all Neuropilin-2 Antibodies
SKU/Catalog Number
PA1771
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neuropilin-2 NRP2 Antibody catalog # PA1771. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuropilin-2 NRP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1771)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NRP2, identical to the related mouse sequence and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1771 is reactive to NRP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
105 kDa
Calculated molecular weight
104859 MW
Background of Neuropilin-2
NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped on 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. Sema3b can act to repulse axons expressing neuropilin-2 and can antagonize the repulsive action of Sema3a on axonal growth cones expressing neuropilin-1. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in Npn2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Npn2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1771 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Testis Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-NRP2 antibody, PA1771, Western blotting
All lanes: Anti NRP2 (PA1771) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Predicted bind size: 105KD
Observed bind size: 105KD
Protein Target Info & Infographic
Gene/Protein Information For NRP2 (Source: Uniprot.org, NCBI)
Gene Name
NRP2
Full Name
Neuropilin-2
Weight
104859 MW
Superfamily
neuropilin family
Alternative Names
Neuropilin-2;Vascular endothelial cell growth factor 165 receptor 2;NRP2;VEGF165R2; NRP2 NP2, NPN2, PRO2714, VEGF165R2 neuropilin 2 neuropilin-2|neuropilin-2a(17)|neuropilin-2a(22)|neuropilin-2b(0)|receptor for VEGF165 and semaphorins class3|vascular endothelial cell growth factor 165 receptor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRP2, check out the NRP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuropilin-2 NRP2 Antibody Picoband® (PA1771)
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No publications found for PA1771
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Customer Q&As
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4 Customer Q&As for Anti-Neuropilin-2 NRP2 Antibody Picoband®
Question
We are currently using anti-NRP2 antibody PA1771 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-NRP2 antibody (PA1771) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-NRP2 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
It shows on the product datasheet, PA1771 anti-NRP2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
Would anti-NRP2 antibody PA1771 work on bovine WB with lower esophagus?
E. Johnson
Verified customer
Asked: 2016-05-26
Answer
Our lab technicians have not validated anti-NRP2 antibody PA1771 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-NRP2 antibody PA1771 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine lower esophagus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-26
Question
Is a blocking peptide available for product anti-NRP2 antibody (PA1771)?
M. Walker
Verified customer
Asked: 2013-07-16
Answer
We do provide the blocking peptide for product anti-NRP2 antibody (PA1771). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-16