Human Granzyme A ELISA Kit PicoKine®

Product Name

Human Granzyme A ELISA Kit PicoKine®

View all Granzyme A ELISA kits

SKU/Catalog Number

EK1162

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Granzyme A ELISA Kit PicoKine® (96 Tests). Quantitate Human GZMA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Granzyme A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1162)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: I29-V262

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK1162 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1162 is reactive to GZMA in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1162 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Granzyme A

Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A.

Kit Components

Catalog Number	Description	Quantity
EK1162-CAP	Anti-Human GZMA Pre-coated 96-well strip microplate	1
EK1162-ST	Human GZMA Standard	2 vials, 10 ng/tube
EK1162-DA	Human GZMA Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.061	0.131	0.208	0.345	0.579	0.936	1.478	2.087

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Granzyme A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Granzyme A in ELISA kits from four different production batches/lots.

Gene/Protein Information For GZMA (Source: Uniprot.Org, NCBI)

Alternative Names

Granzyme A;3.4.21.78;CTL tryptase;Cytotoxic T-lymphocyte proteinase 1;Fragmentin-1;Granzyme-1;Hanukkah factor;H factor;HF;GZMA;CTLA3, HFSP; GZMA CTLA3, HFSP granzyme A granzyme A|CTL tryptase|Cytotoxic T-lymphocyte-associated serine esterase-3|Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)|HF|Hanukah factor serine protease)|cytotoxic T-lymphocyte proteinase 1|fragmentin-1|granzyme 1|granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)|h factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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