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Product Info Summary
| SKU: | A09144-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NRBF2 Antibody Picoband®
SKU/Catalog Number
A09144-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NRBF2 Antibody Picoband® catalog # A09144-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NRBF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09144-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NRBF2 recombinant protein (Position: M1-N287).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09144-2 is reactive to NRBF2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
32.378kDa
Background of NRBF2
Nuclear receptor-binding factor 2 is a protein that in humans is encoded by the NRBF2 gene. Involved in autophagy. Located in cytoplasm. Colocalizes with phosphatidylinositol 3-kinase complex, class III.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09144-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml/ml, Human
Positive Control
WB: human K562 whole cell, human PC-3 whole cell, human U-87MG whole cell
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NRBF2 using anti-NRBF2 antibody (A09144-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRBF2 antigen affinity purified polyclonal antibody (Catalog # A09144-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRBF2 at approximately 38 kDa. The expected band size for NRBF2 is at 32 kDa.
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Figure 2. Flow Cytometry analysis of U20S cells using anti-NRBF2 antibody (A09144-2).
Overlay histogram showing U20S cells stained with A09144-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRBF2 Antibody (A09144-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NRBF2 (Source: Uniprot.org, NCBI)
Gene Name
NRBF2
Full Name
Nuclear receptor-binding factor 2
Weight
32.378kDa
Alternative Names
Tudor domain-containing protein 3; TDRD3 NRBF2 COPR, COPR1, COPR2, NRBF-2 nuclear receptor binding factor 2 nuclear receptor-binding factor 2|comodulator of PPAR and RXR 1|comodulator of PPAR and RXR 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRBF2, check out the NRBF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRBF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NRBF2 Antibody Picoband® (A09144-2)
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