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Product Info Summary
| SKU: | A09144 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NRBF-2 Antibody
SKU/Catalog Number
A09144
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NRBF-2 Antibody catalog # A09144. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NRBF-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09144)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NRBF2. AA range:140-189
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09144 is reactive to NRBF2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
32378 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09144 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using NRBF-2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NRBF2 (Source: Uniprot.org, NCBI)
Gene Name
NRBF2
Full Name
Nuclear receptor-binding factor 2
Weight
32378 MW
Alternative Names
Nuclear receptor-binding factor 2;NRBF-2;Comodulator of PPAR and RXR;NRBF2;COPR; NRBF2 COPR, COPR1, COPR2, NRBF-2 nuclear receptor binding factor 2 nuclear receptor-binding factor 2|comodulator of PPAR and RXR 1|comodulator of PPAR and RXR 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRBF2, check out the NRBF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRBF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NRBF-2 Antibody (A09144)
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No publications found for A09144
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4 Customer Q&As for Anti-NRBF-2 Antibody
Question
Can you help my question with product A09144, anti-NRBF-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09144 anti-NRBF-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-02
Question
Does A09144 anti-NRBF-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
As indicated on the product datasheet, A09144 anti-NRBF-2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-22
Question
We are currently using anti-NRBF-2 antibody A09144 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
The anti-NRBF-2 antibody (A09144) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-20
Question
We want to test anti-NRBF-2 antibody A09144 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NRBF-2 antibody A09144 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Kulkarni
Verified customer
Asked: 2015-11-11
Answer
The products we sell, including anti-NRBF-2 antibody A09144, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-11-11
