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Product Info Summary
| SKU: | A02752-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NR2C2 Antibody Picoband®
SKU/Catalog Number
A02752-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NR2C2 Antibody Picoband® catalog # A02752-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NR2C2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02752-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NR2C2 recombinant protein (Position: R7-L596).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02752-2 is reactive to NR2C2 in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
12252 MW
Background of TR4/NR2C2
Testicular receptor 4 also known as NR2C2 (nuclear receptor subfamily 2, group C, member 2) is a protein that in humans is encoded by the NR2C2 gene. This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02752-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human PC-3 whole cell, human 293T whole cell, monkey COS-7 whole cell, human HEL whole cell, human U20S whole cell, human SH-SY5Y whole cell, rat C6 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell
IHC: human acinic cell carcinoma parotid tissue, human colorectal adenocarcinoma tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NR2C2 using anti-NR2C2 antibody (A02752-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: monkey COS-7 whole cell lysates,
Lane 6: human HEL whole cell lysates,
Lane 7: human U20S whole cell lysates,
Lane 8: human SH-SY5Y whole cell lysates,
Lane 9: rat C6 whole cell lysates,
Lane 10: mouse thymus tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2C2 antigen affinity purified polyclonal antibody (Catalog # A02752-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR2C2 at approximately 68 kDa. The expected band size for NR2C2 is at 68 kDa.
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Figure 2. IHC analysis of NR2C2 using anti-NR2C2 antibody (A02752-2).
NR2C2 was detected in a paraffin-embedded section of human acinic cell carcinoma parotid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NR2C2 Antibody (A02752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of NR2C2 using anti-NR2C2 antibody (A02752-2).
NR2C2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NR2C2 Antibody (A02752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of 293T cells using anti-NR2C2 antibody (A02752-2).
Overlay histogram showing 293T cells stained with A02752-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR2C2 Antibody (A02752-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NR2C2 (Source: Uniprot.org, NCBI)
Gene Name
NR2C2
Full Name
Nuclear receptor subfamily 2 group C member 2
Weight
12252 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; PPBP; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; NAP-2 NR2C2 TAK1, TR4 nuclear receptor subfamily 2 group C member 2 nuclear receptor subfamily 2 group C member 2|Nuclear hormone receptor TR4|orphan nuclear receptor TAK1|orphan nuclear receptor TR4|orphan receptor TR4|testicular nuclear receptor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR2C2, check out the NR2C2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR2C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR2C2 Antibody Picoband® (A02752-2)
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