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Product Info Summary
| SKU: | A02752-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-NR2C2 Antibody (C-term)
SKU/Catalog Number
A02752-1
Size
80 µl
Description
Boster Bio Anti-NR2C2 Antibody (C-term) (Catalog # A02752-1). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NR2C2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02752-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02752-1 is reactive to NR2C2 in Human
Reconstitution
Calculated molecular weight
65414 Da
Background of TR4/NR2C2
Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02752-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.
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NR2C2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NR2C2 antibody detected the NR2C2 protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For NR2C2 (Source: Uniprot.org, NCBI)
Gene Name
NR2C2
Full Name
Nuclear receptor subfamily 2 group C member 2
Weight
65414 Da
Superfamily
nuclear hormone receptor family
Alternative Names
Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, NR2C2, TAK1, TR4 NR2C2 TAK1, TR4 nuclear receptor subfamily 2 group C member 2 nuclear receptor subfamily 2 group C member 2|Nuclear hormone receptor TR4|orphan nuclear receptor TAK1|orphan nuclear receptor TR4|orphan receptor TR4|testicular nuclear receptor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR2C2, check out the NR2C2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR2C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR2C2 Antibody (C-term) (A02752-1)
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