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Product Info Summary
| SKU: | A30570 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-ALS2CR8 Antibody
SKU/Catalog Number
A30570
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ALS2CR8 Antibody (Catalog# A30570). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALS2CR8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30570)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ALS2CR8. Immunogen sequence location: 311-360
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30570 is reactive to CARF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
80698 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30570 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ALS2CR8 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC cells, using ALS2CR8 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CARF (Source: Uniprot.org, NCBI)
Gene Name
CARF
Full Name
Calcium-responsive transcription factor
Weight
80698 MW
Alternative Names
ALS2CR8;CARF;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein;Calcium-response factor;CaRF;Testis development protein NYD-SP24 CARF ALS2CR8, NYD-SP24 calcium responsive transcription factor calcium-responsive transcription factor|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8|amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein|calcium-response factor|testis development protein NYD-SP24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARF, check out the CARF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALS2CR8 Antibody (A30570)
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No publications found for A30570
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Customer Q&As
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7 Customer Q&As for Anti-ALS2CR8 Antibody
Question
I have a question about product A30570, anti-ALS2CR8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30570 anti-ALS2CR8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-16
Question
We are currently using anti-ALS2CR8 antibody A30570 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-ALS2CR8 antibody (A30570) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
Do you have a BSA free version of anti-ALS2CR8 antibody A30570 available?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ALS2CR8 antibody A30570 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-10
Question
Is a blocking peptide available for product anti-ALS2CR8 antibody (A30570)?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do provide the blocking peptide for product anti-ALS2CR8 antibody (A30570). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-24
Question
Please see the WB image, lot number and protocol we used for corpus callosum using anti-ALS2CR8 antibody A30570. Please let me know if you require anything else.
Z. Roberts
Verified customer
Asked: 2018-07-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-20
Question
Will A30570 anti-ALS2CR8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
As indicated on the product datasheet, A30570 anti-ALS2CR8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for corpus callosum using anti-ALS2CR8 antibody A30570. Let me know if you need anything else.
E. Anderson
Verified customer
Asked: 2014-07-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-25
