NADPH oxidase 5 Antibodies

NADPH oxidase 5 (NOX5)

Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and might regulate redox-dependent procedures in lymphocytes and spermatozoa.

May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial production of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

Gene Information

Human
Gene Name:	NOX5
Uniprot:	Q96PH1
Entrez:	79400

Family

Belongs to:
No superfamily

Alternative Names

EC 1.6.3.-; MGC149776; MGC149777; NADPH oxidase 5; NADPH oxidase, EF-hand calcium binding domain 5; NOX5A; NOX5B

Molecular Weight

Mass (kDA):
86.439 kDA

Genomic Context

Human
Location:	15q23
Sequence:	15; NC_000015.10 (68930525..69062762)

Tissue Specificity

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).

Subcellular Localizations

Membrane; Multi-pass membrane protein.; [Isoform v2]: Endoplasmic reticulum.; [Isoform v5]: Endoplasmic reticulum.

Diseases

• Adenocarcinoma
• Esophageal Adenocarcinoma
• Esophageal Neoplasms
• Barrett Esophagus
• Malignant Neoplasms
• Neoplasms
• Inflammation
• Gastroesophageal Reflux Disease
• Cell Transformation, Neoplastic
• Atherosclerosis
• Carcinoma
• Injury To Kidney
• Dysplasia

• Tumor Angiogenesis
• Malignant Paraganglionic Neoplasm
• Hypertrophy
• Coronary Artery Disease
• Cardiovascular Diseases
• Hypothyroidism
• Hypertensive Disease

Pathways

• Cell Proliferation
• Localization
• Transport
• Electron Transport
• Respiratory Burst
• Pathogenesis
• Aging
• Cell Cycle
• Senescence
• Acrosome Reaction
• Cellular Localization
• Cell Growth
• Angiogenesis

• Protein Phosphorylation
• Cell Adhesion
• Programmed Cell Death
• Fertilization
• Proteolysis
• Cytokine Secretion
• Muscle Contraction

PTMs

• Phosphorylation
• Nitration
• Nitrosylation

• Cleavage
• Oxidation
• Methylation

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NOX5

Best Uses Of The NOX5 Marker With Boster Bio

To achieve the best results, a guide on optimizing your experiments with Boster Bio is crucial. This guide will provide solutions to many common queries, and will help you design the best experiments and improve your results. Every researcher is bound to make mistakes at some time or another. Fortunately, there are many reasons for error, and having the right controls can eliminate a lot of them. Troubleshooting guides can help you determine the root of any trouble you are having and resolve the problem.

Boster's primary antibodies

NOX5 is a molecule which can be expressed in several cell types that include blood vessels of humans. The NOX5 protein encoded in isoforms could be utilized for a variety of purposes such as the generation of superoxide. It functions as an H+ channel but it is Ca2+-dependent. Before, scientists didn't understand the function of NOX5 in the vascular cells. Interestingly, Kamiguti et al. discovered that NOX5 was a flavin-containing Ca2+ dependent oxidase.

The expression of NOX5 is increased in individuals with atherosclerosis (17). Studies in mice and rats have revealed that OLs express NOX5. It has been confirmed that ECs and HVSMCs have all five splice variants. While NOX5 is not found in rodent genomes it has been established that all five splice variations produce ROS. Interestingly, some reports have proven that NOX5-e can be active in the basal levels and contributes to the proliferative status of the esophageal carcinoma.

Human blood vessels contain two versions of the NOX5 protein Nox5-a and Nox5 b. The latter is an mRNA sequence that is conserved. cDNA was synthesized by reverse transcription using oligo-dT primers. Additionally, RNA was extracted from the mammary artery in humans. It was treated with LacZ. After treatment the cells were harvested with 0.3 percent trypsin and then put back into 1 ml of fresh media. After an interval of at least 24 hours, the cells were counted manually using a hemocytometer.

Proinflammatory and vasoactive treatment increase the expression of the Nox5-e gene. This was determined by treating HVSMCs with endothelin-1 as well as tumor necrosis factors-a for 24 hrs. Then, real-time PCR was used to determine the mRNA levels. The results were then normalized to 18S.

The anti-NOX5 polyclonal antibodies detects total Nox5 expression. It is also known that Nox5 activates ERK when intracellular Ca2+ levels increase. Nox5 is extremely expressed in human vascular cells. This polyclonal antibody was created to identify these cells. These results suggest that NOX5 is an endothelial signaling protein. cell membranes.

High-affinity primary antibodies

Recombinant Fabs are generated from large naive antibody libraries. Recombinant antibodies can provide several advantages over monoclonal antigens. In the laboratory, Fab generation can provide greater control over selection parameters and allows access to a greater variety of antigens, and is more efficient than traditional selection of bacterial species. Recombinant antibodies are not tested against short linear peptides in E. coli. More studies are required to determine if recombinant antibodies can be used for immunocapture small linear peptides.

Immunoblots using a Nox5 peptide showed that the protein corresponding to PBR-N in prostate cancer cells and hairy leukemia cells binds to the resin. Antibodies against mutations in PBR-N bound the resin. Non-binding proteins were removed by 17 percent SDS–PAGE. Finally, the Recombinant protein was identified using an anti-hexahistidine antigen.

Multiple peptides may be made from the same peptides, using the Fabs. The Fabs were constructed in one stage and then used for the peptide immunomRM assays. The peptides were used as immunogens and the Fabs were compared to conventional anti-peptide antibodies. These recombinant antibody were successful in the immuno-capture tests, and the best Fab was isolated from the ADAM17 peptide.

Two poly-basic areas of Nox5 are preserved in the Nox5 polypeptide. They are located between the hand of the EF and the initial transmembrane region. PtdIns(4,5)P2 regulates the localization of Nox5 within the plasma membrane. The PtdIns (4,5)P2 region that binds to phosphate is responsible for its localization. However, PtdIns-P2 also contributes to the localization of the protein within the plasma membrane.

The Nox5 protein was identified by TCEP-based peptides. The TCEP is a stronger reduction agent than dithiothreitol however dithiothreitol and both TCEP were less effective in reducing agents. Three independent experiments confirmed this conclusion. The use of the NOX5 marker has been proven to be effective in detecting various types of targets in a variety.

PtdIns(4) P-5K and p22phox have been used as reagents in apoptosis. The PtdIns(4)P-5K protein kinase enzyme was able to increase Nox5 expression on the cell surface but did not boost the total protein levels. PtdIns(4)P-5K inhibited the activity of Nox5 in fibroblasts , and increased the level of NOX5 expression in these cells.

Troubleshooting

Nox5 is a protein that is expressed by cells of the endothelial or vascular smooth muscle types. Nox5 isn't expressed in macrophages, T-cells , or fibroblasts. This protein is essential in the development and maintenance of the immune system. However, it might not be active in peripheral tissues. Understanding the function of the NOX5 marker can help to diagnose the issue.

Optimization

The most important step in success in research is optimizing the effectiveness of your research. It is possible to improve the results of your research by employing the right tools and techniques. The following guidelines can help you optimize your experiment. In addition to providing helpful advice and guidelines, Boster offers a comprehensive collection of technical sources, including blogs and information about diseases. The lysis buffers provided by Boster ensure that your experiments achieve the most effective results and lessen the intensity of cross-linking.