Anti-NADPH oxidase 1 NOX1 Antibody Picoband®

Product Name

Anti-NADPH oxidase 1 NOX1 Antibody Picoband®

View all NOX1 Antibodies

SKU/Catalog Number

PA1739

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NADPH oxidase 1 NOX1 Antibody catalog # PA1739. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADPH oxidase 1 NOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1739)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOX1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1739 is reactive to NOX1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

65 kDa

Calculated molecular weight

64871 MW

Background of NOX1

NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1739 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Whole Cell, MCF-7 Whole Cell
IHC: Human Lung Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For NOX1 (Source: Uniprot.org, NCBI)

Alternative Names

NADPH oxidase 1;NOX-1;1.-.-.-;Mitogenic oxidase 1;MOX-1;NADH/NADPH mitogenic oxidase subunit P65-MOX;NOH-1;NOX1;MOX1, NOH1; NOX1 GP91-2, MOX1, NOH-1, NOH1 NADPH oxidase 1 NADPH oxidase 1|NADH/NADPH mitogenic oxidase subunit P65-MOX|NADPH oxidase homolog-1|mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)|mitogenic oxidase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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