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Product Info Summary
| SKU: | A04335 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NOP56 Antibody
SKU/Catalog Number
A04335
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NOP56 Antibody catalog # A04335. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOP56 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04335)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG1
Immunogen
Synthesized peptide derived from NOP56 at AA range: 191-240
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04335 is reactive to NOP56 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
66050 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04335 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
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Western Blot (WB) analysis of HepG2 HeLa cells using NOP56 Polyclonal antibody diluted at 1:1000.
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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For NOP56 (Source: Uniprot.org, NCBI)
Gene Name
NOP56
Full Name
Nucleolar protein 56
Weight
66050 MW
Superfamily
NOP5/NOP56 family
Alternative Names
Nucleolar protein 56;Nucleolar protein 5A;NOP56;NOL5A; NOP56 NOL5A, SCA36 NOP56 ribonucleoprotein nucleolar protein 56|NOP56 ribonucleoprotein homolog|nucleolar protein 5A (56kDa with KKE/D repeat)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOP56, check out the NOP56 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOP56: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOP56 Antibody (A04335)
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No publications found for A04335
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Customer Q&As
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13 Customer Q&As for Anti-NOP56 Antibody
Question
I see that the anti-NOP56 antibody A04335 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-22
Question
We are interested in to test anti-NOP56 antibody A04335 on rat prostate cancer for research purposes, then I may be interested in using anti-NOP56 antibody A04335 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The products we sell, including anti-NOP56 antibody A04335, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-21
Question
Will anti-NOP56 antibody A04335 work for WB with prostate cancer?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
According to the expression profile of prostate cancer, NOP56 is highly expressed in prostate cancer. So, it is likely that anti-NOP56 antibody A04335 will work for WB with prostate cancer.
Boster Scientific Support
Answered: 2019-10-22
Question
Is this A04335 anti-NOP56 antibody reactive to the isotypes of NOP56?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The immunogen of A04335 anti-NOP56 antibody is Synthesized peptide derived from NOP56 at AA range: 191-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-02
Question
Do you have a BSA free version of anti-NOP56 antibody A04335 available?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NOP56 antibody A04335 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-07
Question
See attached the WB image, lot number and protocol we used for prostate cancer using anti-NOP56 antibody A04335. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-28
Question
We are currently using anti-NOP56 antibody A04335 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
The anti-NOP56 antibody (A04335) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-09
Question
Will A04335 anti-NOP56 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
It shows on the product datasheet, A04335 anti-NOP56 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-20
Question
Is a blocking peptide available for product anti-NOP56 antibody (A04335)?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
We do provide the blocking peptide for product anti-NOP56 antibody (A04335). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-15
Question
My question regarding product A04335, anti-NOP56 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Li
Verified customer
Asked: 2015-12-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04335 anti-NOP56 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-12-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate cancer using anti-NOP56 antibody A04335. Let me know if you need anything else.
N. Dhar
Verified customer
Asked: 2015-09-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-18
Question
I was wanting to use your anti-NOP56 antibody for WB for rat prostate cancer on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat prostate cancer identification?
H. Gonzalez
Verified customer
Asked: 2015-05-20
Answer
As indicated on the product datasheet, A04335 anti-NOP56 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat prostate cancer in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-20
Question
Would anti-NOP56 antibody A04335 work on primate IHC with liver?
J. Jones
Verified customer
Asked: 2015-03-30
Answer
Our lab technicians have not tested anti-NOP56 antibody A04335 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NOP56 antibody A04335 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-30
