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Product Info Summary
| SKU: | A11010 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-KLHL1 Monoclonal Antibody
SKU/Catalog Number
A11010
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KLHL1 Monoclonal Antibody catalog # A11010. Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLHL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11010)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3A8
Isotype
IgG1
Immunogen
Purified recombinant fragment of human KLHL1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11010 is reactive to KLHL1 in Human
Reconstitution
Calculated molecular weight
82680 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11010 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis using KLHL1 monoclonal antibody against KLHL1 recombinant protein.
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Immunofluorescence analysis of NIH/3T3 cells using KLHL1 monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Protein Target Info & Infographic
Gene/Protein Information For KLHL1 (Source: Uniprot.org, NCBI)
Gene Name
KLHL1
Full Name
Kelch-like protein 1
Weight
82680 MW
Alternative Names
Kelch-like protein 1;KLHL1;KIAA1490; KLHL1 MRP2 kelch like family member 1 kelch-like protein 1|Mayven-related protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLHL1, check out the KLHL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLHL1 Monoclonal Antibody (A11010)
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No publications found for A11010
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-KLHL1 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for neocortex using anti-KLHL1 Monoclonal antibody A11010. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
Would A11010 anti-KLHL1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
It shows on the product datasheet, A11010 anti-KLHL1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neocortex using anti-KLHL1 Monoclonal antibody A11010. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-KLHL1 Monoclonal antibody A11010 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The anti-KLHL1 Monoclonal antibody (A11010) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
Question
I was wanting to use your anti-KLHL1 Monoclonal antibody for IF for human neocortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human neocortex identification?
L. Jha
Verified customer
Asked: 2018-04-10
Answer
It shows on the product datasheet, A11010 anti-KLHL1 Monoclonal antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human neocortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-10
Question
I see that the anti-KLHL1 Monoclonal antibody A11010 works with IF, what is the protocol used to produce the result images on the product page?
J. Carter
Verified customer
Asked: 2017-07-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-10
Question
We are interested in to test anti-KLHL1 Monoclonal antibody A11010 on human neocortex for research purposes, then I may be interested in using anti-KLHL1 Monoclonal antibody A11010 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Edwards
Verified customer
Asked: 2014-01-10
Answer
The products we sell, including anti-KLHL1 Monoclonal antibody A11010, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-10
