Anti-NOL10 Antibody Picoband™

Product Name

Anti-NOL10 Antibody Picoband™

View all NOL10 Antibodies

SKU/Catalog Number

A14358-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOL10 Antibody Picoband™ catalog # A14358-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NOL10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14358-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NOL10 recombinant protein (Position: Y73-D497).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14358-3 is reactive to NOL10 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

80 kDa

Calculated molecular weight

64099 MW

Background of NOL10

By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14358-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NOL10 using anti-NOL10 antibody (A14358-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human U937 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL10 antigen affinity purified polyclonal antibody (Catalog # A14358-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL10 at approximately 80 kDa. The expected band size for NOL10 is at 80 kDa.

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Figure 2. IF analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4) and anti-NOL10 antibody (A14358-3). Tubulin beta was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Tubulin beta Antibody (M05613-4) and rabbit anti-NOL10 antibody (A14358-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) and DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For NOL10 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ13938; FLJ14075; H_NH0074G24.1; nucleolar protein 10; polyglutamine binding protein 5; PQBP5 NOL10 PQBP5 nucleolar protein 10 nucleolar protein 10|H_NH0074G24.1|polyglutamine binding protein 5

For more info on NOL10, check out the NOL10 Infographic

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In this infographic, you will see the following information for NOL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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