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Product Info Summary
| SKU: | M02250 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Nogo Receptor Rabbit Monoclonal Antibody
View all Nogo Receptor/NgR Antibodies
SKU/Catalog Number
M02250
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Nogo Receptor Rabbit Monoclonal Antibody catalog # M02250. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nogo Receptor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02250)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22R65
Isotype
IgG
Immunogen
A synthesized peptide derived from human Nogo Receptor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02250 is reactive to RTN4R in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
51,70 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02250 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:50
Positive Control
WB: human HepG2 whole cell, human U20S whole cell, human SH-SY5Y whole cell, human U251 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Nogo Receptor using anti-Nogo Receptor antibody (M02250).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo Receptor antigen affinity purified monoclonal antibody (Catalog # M02250) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo Receptor at approximately 70 kDa. The expected band size for Nogo Receptor is at 51,70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RTN4R (Source: Uniprot.org, NCBI)
Gene Name
RTN4R
Full Name
Reticulon-4 receptor
Weight
51,70 kDa
Superfamily
Nogo receptor family
Alternative Names
Oligodendrocyte transcription factor 2;Oligo2;Class B basic helix-loop-helix protein 1;bHLHb1;Class E basic helix-loop-helix protein 19;bHLHe19;Protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;OLIG2;BHLHB1, BHLHE19, PRKCBP2, RACK17; RTN4R NGR, NOGOR reticulon 4 receptor reticulon-4 receptor|Nogo-66 receptor|UNQ330/PRO526|nogo receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RTN4R, check out the RTN4R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RTN4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nogo Receptor Rabbit Monoclonal Antibody (M02250)
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