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Product Info Summary
| SKU: | A00087 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-NOD2 (Card15) Antibody
SKU/Catalog Number
A00087
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-NOD2 (Card15) Antibody (Catalog# A00087). Tested in WB, IHC, IP application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-NOD2 (Card15) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00087)
Host
Rabbit
Contents
50 μl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide of human NOD2/CARD15 (amino acids 582-600 LGFLVRAKGVVPGSTAPLE)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
(ELISA)Recombinant canine CCL3 Weak
(ELISA)Recombinant equine CCL3 None
(ELISA)Recombinant mouse CCL3 None
(ELISA)Recombinant rabbit CCL3 None
Reactive Species
A00087 is reactive to NOD2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
115283 MW
Background of NOD2
ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00087 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using primary antibody dilution ratio of 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.
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Western blot analysis of NOD2 using primary antibody dilution ratio of 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.
Protein Target Info & Infographic
Gene/Protein Information For NOD2 (Source: Uniprot.org, NCBI)
Gene Name
NOD2
Full Name
Nucleotide-binding oligomerization domain-containing protein 2
Weight
115283 MW
Alternative Names
Nucleotide-binding oligomerization domain-containing protein 2;Caspase recruitment domain-containing protein 15;Inflammatory bowel disease protein 1;NOD2;CARD15, IBD1; NOD2 ACUG, BLAU, BLAUS, CARD15, CD, CLR16.3, IBD1, NLRC2B, PSORAS1, YAOS, NOD2 nucleotide binding oligomerization domain containing 2 nucleotide-binding oligomerization domain-containing protein 2|NLR family, CARD domain containing 2|NOD-like receptor C2|caspase recruitment domain family, member 15|caspase recruitment domain protein 15|caspase recruitment domain-containing protein 15|inflammatory bowel disease protein 1|nucleotide-binding oligomerization domain 2|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOD2, check out the NOD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOD2 (Card15) Antibody (A00087)
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No publications found for A00087
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-NOD2 (Card15) Antibody
Question
Is this A00087 anti-NOD2 (Card15) antibody reactive to the isotypes of NOD2?
E. Williams
Verified customer
Asked: 2018-10-03
Answer
The immunogen of A00087 anti-NOD2 (Card15) antibody is A synthetic peptide of human NOD2/CARD15 (amino acids 582-600 LGFLVRAKGVVPGSTAPLE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-03
Question
We are currently using anti-NOD2 (Card15) antibody A00087 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
E. Li
Verified customer
Asked: 2017-09-19
Answer
The anti-NOD2 (Card15) antibody (A00087) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-19
Question
I was wanting to use your anti-NOD2 (Card15) antibody for IP for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
G. Dhar
Verified customer
Asked: 2016-03-21
Answer
It shows on the product datasheet, A00087 anti-NOD2 (Card15) antibody has been tested for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-21
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-NOD2 (Card15) antibody A00087. Please let me know if you require anything else.
K. Li
Verified customer
Asked: 2013-04-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-26
