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Product Info Summary
| SKU: | A01460-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GALT Antibody Picoband®
SKU/Catalog Number
A01460-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GALT Antibody Picoband® catalog # A01460-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GALT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01460-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human GALT recombinant protein (Position: Q188-A379).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01460-1 is reactive to GALT in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
43363 MW
Background of GALT
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01460-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 cell, human MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GALT using anti-GALT antibody (A01460-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 cell lysates,
Lane 2: human MCF-7 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALT antigen affinity purified polyclonal antibody (Catalog # A01460-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALT at approximately 48KD. The expected band size for GALT is at 43KD.
Protein Target Info & Infographic
Gene/Protein Information For GALT (Source: Uniprot.org, NCBI)
Gene Name
GALT
Full Name
Galactose-1-phosphate uridylyltransferase
Weight
43363 MW
Superfamily
galactose-1-phosphate uridylyltransferase type 1 family
Alternative Names
Galactose-1-phosphate uridylyltransferase;Gal-1-P uridylyltransferase;2.7.7.12;UDP-glucose--hexose-1-phosphate uridylyltransferase;GALT; GALT galactose-1-phosphate uridylyltransferase galactose-1-phosphate uridylyltransferase|UDP-glucose--hexose-1-phosphate uridylyltransferase|gal-1-P uridylyltransferase|galactose-1-phosphate uridyl transferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALT, check out the GALT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GALT Antibody Picoband® (A01460-1)
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No publications found for A01460-1
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Customer Q&As
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7 Customer Q&As for Anti-GALT Antibody Picoband®
Question
Is this A01460-1 anti-GALT antibody reactive to the isotypes of GALT?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The immunogen of A01460-1 anti-GALT antibody is E. coli-derived human GALT recombinant protein (Position: Q188-A379). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-06
Question
I see that the anti-GALT antibody A01460-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-10
Question
Is a blocking peptide available for product anti-GALT antibody (A01460-1)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do provide the blocking peptide for product anti-GALT antibody (A01460-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-01
Question
I am looking for to test anti-GALT antibody A01460-1 on mouse right lobe of liver for research purposes, then I may be interested in using anti-GALT antibody A01460-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The products we sell, including anti-GALT antibody A01460-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-21
Question
Will A01460-1 anti-GALT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
It shows on the product datasheet, A01460-1 anti-GALT antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-11
Question
Please see the WB image, lot number and protocol we used for right lobe of liver using anti-GALT antibody A01460-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-01
Question
We are currently using anti-GALT antibody A01460-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
C. Johnson
Verified customer
Asked: 2016-09-27
Answer
The anti-GALT antibody (A01460-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-27
