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Product Info Summary
| SKU: | A00087-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NOD2 Antibody
SKU/Catalog Number
A00087-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NOD2 Antibody (Catalog # A00087-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
NOD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NOD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00087-1)
Host
Rabbit
Contents
NOD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NOD2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2. The immunogen is located within the first 50 amino acids of NOD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of NOD2 are known to exist; this antibody will only detect the longest isoform. NOD2 has no cross-reaction with NOD1.
Reactive Species
A00087-1 is reactive to NOD2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
115283 MW
Background of NOD2
Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00087-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NOD2 antibody can be used for detection of NOD2 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NOD2 in human lymph node tissue lysate with NOD2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of NOD2 in Jurkat cells with NOD2 antibody at 5 μg/mL.
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Immunofluorescence of NOD2 in Jurkat cells with NOD2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NOD2 (Source: Uniprot.org, NCBI)
Gene Name
NOD2
Full Name
Nucleotide-binding oligomerization domain-containing protein 2
Weight
115283 MW
Alternative Names
CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15 NOD2 ACUG, BLAU, BLAUS, CARD15, CD, CLR16.3, IBD1, NLRC2B, PSORAS1, YAOS, NOD2 nucleotide binding oligomerization domain containing 2 nucleotide-binding oligomerization domain-containing protein 2|NLR family, CARD domain containing 2|NOD-like receptor C2|caspase recruitment domain family, member 15|caspase recruitment domain protein 15|caspase recruitment domain-containing protein 15|inflammatory bowel disease protein 1|nucleotide-binding oligomerization domain 2|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOD2, check out the NOD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOD2 Antibody (A00087-1)
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