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Product Info Summary
| SKU: | A15072 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NMS/Neuromedin S Antibody
View all Neuromedin S Antibodies
SKU/Catalog Number
A15072
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NMS/Neuromedin S Antibody (Catalog # A15072). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMS/Neuromedin S Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15072)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NMS.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15072 is reactive to NMS in Human
Reconstitution
Calculated molecular weight
17731 MW
Background of Neuromedin S
Implicated in the regulation of circadian rhythms through autocrine and/or paracrine actions By similarity.
Mori K., EMBO J. 24:325-335(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15072 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from MCF-7 cells, 293 cells, HUVEC cells, HepG2 cells and Jurkat cells, using NMS antibody A15072.
Protein Target Info & Infographic
Gene/Protein Information For NMS (Source: Uniprot.org, NCBI)
Gene Name
NMS
Full Name
Neuromedin-S
Weight
17731 MW
Superfamily
NmU family
Alternative Names
Neuromedin-S;NMS; NMS neuromedin S neuromedin-S|neuromedin S precursor-related peptide/neuromedin S preproprotein|prepro-NMS
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NMS, check out the NMS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NMS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMS/Neuromedin S Antibody (A15072)
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No publications found for A15072
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-NMS/Neuromedin S Antibody
Question
I was wanting to use your anti-NMS/Neuromedin S antibody for WB for human hypothalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human hypothalamus identification?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
You can see on the product datasheet, A15072 anti-NMS/Neuromedin S antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human hypothalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
Will A15072 anti-NMS/Neuromedin S antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
As indicated on the product datasheet, A15072 anti-NMS/Neuromedin S antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-18
Question
I see that the anti-NMS/Neuromedin S antibody A15072 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hypothalamus using anti-NMS/Neuromedin S antibody A15072. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-16
Question
We are currently using anti-NMS/Neuromedin S antibody A15072 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
B. Wu
Verified customer
Asked: 2018-12-12
Answer
The anti-NMS/Neuromedin S antibody (A15072) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-12
Question
I have a question about product A15072, anti-NMS/Neuromedin S antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15072 anti-NMS/Neuromedin S antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-28
Question
you antibody to test anti-NMS/Neuromedin S antibody A15072 on human hypothalamus for research purposes, then I may be interested in using anti-NMS/Neuromedin S antibody A15072 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Jha
Verified customer
Asked: 2017-11-21
Answer
The products we sell, including anti-NMS/Neuromedin S antibody A15072, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-21
