Anti-RPL28/Ribosomal Protein L28 Antibody

Product Name

Anti-RPL28/Ribosomal Protein L28 Antibody

View all RPL28 Antibodies

SKU/Catalog Number

A10323

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RPL28/Ribosomal Protein L28 Antibody (Catalog # A10323). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPL28/Ribosomal Protein L28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10323)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RPL28.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10323 is reactive to RPL28 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

15748 MW

Background of RPL28

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Frigerio J.-M., Biochim. Biophys. Acta 1262:64-68(1995).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10323 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For RPL28 (Source: Uniprot.org, NCBI)

Alternative Names

60S ribosomal protein L28;RPL28; RPL28 L28 ribosomal protein L28 60S ribosomal protein L28|large ribosomal subunit protein eL28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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