Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody

Product Name

Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody

View all NHERF-1 Antibodies

SKU/Catalog Number

M02427-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody (Catalog# M02427-1). Tested in IP, WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02427-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EBP-10

Isotype

Mouse IgG1 kappa

Immunogen

Bacterially produced recombinant full-length human NHERF1. The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02427-1 is reactive to SLC9A3R1 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

38868 MW

Background of NHERF-1

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M02427-1 is guaranteed for IP, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 5 μg/ml.
Western blotting: 2 μg/ml, reducing conditions, positive control: MCF-7 cell line

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For SLC9A3R1 (Source: Uniprot.org, NCBI)

Alternative Names

EBP50, NPHLOP2, SLC9A3R1, SR1 SLC9A3R1 EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2 SLC9A3 regulator 1 Na(+)/H(+) exchange regulatory cofactor NHE-RF1|Na+/H+ exchange regulatory co-factor|ezrin-radixin-moesin binding phosphoprotein-50|regulatory cofactor of Na(+)/H(+) exchanger|solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis