Anti-Lumican Antibody Picoband™

Product Name

Anti-Lumican Antibody Picoband™

View all Lumican Antibodies

SKU/Catalog Number

PB9662

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lumican Antibody Picoband™ catalog # PB9662. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lumican Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9662)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Lumican recombinant protein (Position: I68-N338). Mouse Lumican shares 88.9% and 98.2% amino acid (aa) sequence identity with human and rat Lumican, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9662 is reactive to LUM in Mouse

Applications

PB9662 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

38265 MW

Background of Lumican

Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Lumican using anti-Lumican antibody (PB9662).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Mouse Testis Tissue Lysate at 50ug,
Lane 2: Mouse Thymus Tissue Lysate at 50ug,
Lane 3: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # PB9662) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 60 kDa. The expected band size for Lumican is at 38 kDa.

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Figure 2. IHC analysis of Lumican using anti-Lumican antibody (PB9662).
Lumican was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lumican Antibody (PB9662) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For LUM (Source: Uniprot.org, NCBI)

Alternative Names

KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican LUM LDC, SLRR2D lumican lumican|KSPG lumican|epididymis secretory sperm binding protein|keratan sulfate proteoglycan lumican|lumican proteoglycan

For more info on LUM, check out the LUM Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LUM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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