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Product Info Summary
| SKU: | PB9662 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Lumican Antibody Picoband®
SKU/Catalog Number
PB9662
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lumican Antibody Picoband® catalog # PB9662. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lumican Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9662)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Lumican recombinant protein (Position: I68-N338). Mouse Lumican shares 88.9% and 98.2% amino acid (aa) sequence identity with human and rat Lumican, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9662 is reactive to Lum in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
38265 MW
Background of Lumican
Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9662 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Testis Tissue, Mouse Thymus Tissue, NIH3T3 Whole Cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Lumican using anti-Lumican antibody (PB9662).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Mouse Testis Tissue Lysate at 50ug,
Lane 2: Mouse Thymus Tissue Lysate at 50ug,
Lane 3: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # PB9662) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 60 kDa. The expected band size for Lumican is at 38 kDa.
Click image to see more details
Figure 2. IHC analysis of Lumican using anti-Lumican antibody (PB9662).
Lumican was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lumican Antibody (PB9662) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Lum (Source: Uniprot.org, NCBI)
Gene Name
Lum
Full Name
Lumican
Weight
38265 MW
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
Lumican;Keratan sulfate proteoglycan lumican;KSPG lumican;Lum;Lcn, Ldc; LUM LDC, SLRR2D lumican lumican|KSPG lumican|epididymis secretory sperm binding protein|keratan sulfate proteoglycan lumican|lumican proteoglycan
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lum, check out the Lum Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lum: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lumican Antibody Picoband® (PB9662)
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Customer Q&As
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1 Customer Q&As for Anti-Lumican Antibody Picoband®
Question
We are currently using anti-Lumican antibody PB9662 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The anti-Lumican antibody (PB9662) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
