SLC9A3R1 Antibodies

Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (SLC9A3R1)

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to connect them to the actin cytoskeleton and to regulate their surface expression. Was known to play a role in the regulation of the activity and subcellular location of SLC9A3.

Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May improve Wnt signaling.

Gene Information

Human
Gene Name:	SLC9A3R1
Uniprot:	O14745
Entrez:	9368

Family

Belongs to:
No superfamily

Alternative Names

EBP50NHERF-1; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; NHERFNPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; sodium/hydrogen exchanger regulatory factor 1; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute car

Molecular Weight

Mass (kDA):
38.868 kDA

Genomic Context

Human
Location:	17q25.1
Sequence:	17; NC_000017.11 (74748628..74769353)

Tissue Specificity

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

Subcellular Localizations

Cytoplasm. Apical cell membrane. Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.

Diseases

• Psoriasis
• Arthritis
• Arthritis, Psoriatic
• Asthma
• Crohn Disease
• Dermatitis, Atopic
• Lupus Erythematosus, Systemic
• Rheumatoid Arthritis
• Dermatitis
• Diabetes Mellitus
• Autoimmune Diseases
• Autoimmune Reaction

• Diabetes Mellitus, Insulin-dependent
• Autoimmunity
• Sclerosis
• Rhinorrhea
• Cystic Fibrosis
• Polyps
• Malignant Neoplasm Of Liver

Interacting Proteins

• ADRB2
• CFTR
• EZR
• MSN
• P2RY1

• PDGFRB
• PHLPP1
• PHLPP2
• PTEN
• Rdx

Pathways

• Pathogenesis
• Transport
• Programmed Cell Death
• Osteoblast Differentiation
• Osteoblast Development
• Cell Development
• Fertilization
• Localization
• Platelet Aggregation
• Urea Cycle

• Cell Death
• Sperm Capacitation
• Phototransduction
• Wound Healing

PTMs

• Phosphorylation

Research Areas

• Cell Biology
• Cellular Markers
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLC9A3R1

Best Uses Of The SLC9A3R1 Marker

SLC9A3R1 is a protein which is involved in the synthesis of chimeric antigen receptors, like IL-12. The protein is known to be abundant in a variety of organs and tissues and is crucial for a variety of biomedical research applications. Boster Bio produces several products to aid in this research. PICOKINE(tm) ELISA kits and Autoradiography films are among the ones available. These kits can be used to detect antibodies that are bound.

Boster Bio's research-based primary antibodies

Antibodies against the EBP50 protein (SLC9A3R1) are a multi-faceted instrument that can detect the activities of proteins involved in the biosynthesis of steroid. Boster Bio A02427 & A02428 have antibodies against SLC9A3R1. Researchers can conduct a variety of analyses because each antibody reacts with one or more of these proteins.

The SLC9A3R1 gene can be found in the cytoplasmic cells of breast cancer cells. It is involved in autophagy. It is a stimulator of the PTEN–PI3K–AKT1 pathways However, it is found primarily in the cytoplasm. Subcellular fractions of breast cancer cells were used for immunoblotting tests. Controls were ACTB and LMNA. Cells were fixed with 4percent paraformaldehyde. They were stained with fluorochromes. They were then images were taken of conforomes.

The SLC9A3R1 protein hinders the ubiquitin-dependent degradation of BECN1. The deletion of the BCL2-binding domain of BECN1 decreased its binding to SLC9A3R1. The overexpression of SLC9A3R1 decreases ubiquitination. Therefore, BECN1 is inhibited by SLC9A3R1-HA-antibodies.

PICOKINE(tm) ELISA kits

Picokine(tm), ELISA kits are based upon a unique technology that increases the affinity of antibodies to capture and decreases non-specific binding. This results in single-digit picogram that have a high sensitivity as well as a low background. The kits are able to be used to verify the sensitivity of a variety of samples. They contain enough reagents for up to 96 tests. The Picokine(tm) ELISA kits are available from tebu-bio.

Film on autoradiography

The SLC9A3R1 enzyme marker regulates the activities of the SLC9 transporter. Scientists using this enzyme can observe molecular and cellular processes. The enzyme can also be detected with ELISA methods. It has proved to be a useful tool in research on neuroscience, cancer, developmental biology, and inflammation. To identify this marker, the company has developed a variety of ELISA kits. The kits offer picogram level sensitiveness and are suitable to use in a wide range of research applications.

Western blot antibody detection methods

The primary function of SLC9A3R1 Western blot antibody is to detect and characterize the protein in samples. It is used for many different purposes, including Alzheimer's research. This technique detects a specific protein that is present in the normal brain and the affected regions. The Western blot also confirms a positive ELISA test.

SLC9A3R1 could be detected in Western blots after centrifuging the lysates of different cell cultures at 15,000xg for 15 minutes at 4degC. The supernatants were then precleared using 5 milliliters of mouse IgG beads following centrifugation. Then, a 25 mL antibody was incubated with the sample, and the results were observed the following day. The membrane was rinsed three times using ice-cold 0.1% Tween 20, before being rinsed with PBS. Then the membrane was stained by the secondary antibody. The sample should be incubated at room temperature for at least an hour.

The primary antibody for SLC9A3R1 has detected endogenous Carcinoembryonic antigen (CEA) protein. This antibody is part of the Picoband(tm). This antibody reacts with Mouse and Rat, Human, as well as Mouse. It exhibits excellent cross-reactivity, and is ideal for research projects in which the protein is expressed in a variety of tissues.

The two most commonly used methods to detect the SLC9A3R1 protein is through immunohistochemistry and fluorescent. Chemiluminescence employs a second antibody conjugated with an molecule that is fluorescent. The amount of light emitted is proportional to the amount of protein on the membrane. These methods permit researchers to multiply secondary antibodies. This method is the most widely used because it permits researchers to combine two antibodies at the same time.

An immunoblot can be used to identify specific proteins in the samples. It involves two steps, SDS-polyacrylamide Gel Electrophoresis (Pbblotting) and protein blotting. Here, we describe the two methods of protein detection. Let's take a closer review of each. Once you've determined which method is best for your research, it's time to download your data!