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Product Info Summary
| SKU: | PA2276 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase Mer MERTK Antibody Picoband®
SKU/Catalog Number
PA2276
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tyrosine-protein kinase Mer MERTK Antibody catalog # PA2276. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase Mer MERTK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2276)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MERTK, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2276 is reactive to MERTK in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
110249 MW
Background of Mer
Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. This gene is also very important in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2276 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, monkey kidney tissue, rat testis tissue, mouse testis tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MERTK using anti-MERTK antibody (PA2276).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: monkey kidney tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # PA2276) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 110 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (PA2276).
Overlay histogram showing HepG2 cells stained with PA2276 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (PA2276, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MERTK (Source: Uniprot.org, NCBI)
Gene Name
MERTK
Full Name
Tyrosine-protein kinase Mer
Weight
110249 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Mer;2.7.10.1;Proto-oncogene c-Mer;Receptor tyrosine kinase MerTK;MERTK;MER; MERTK MER, RP38, Tyro12, c-Eyk, c-mer MER proto-oncogene, tyrosine kinase tyrosine-protein kinase Mer|MER receptor tyrosine kinase|STK kinase|c-mer proto-oncogene tyrosine kinase|proto-oncogene c-Mer|receptor tyrosine kinase MerTK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MERTK, check out the MERTK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MERTK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase Mer MERTK Antibody Picoband® (PA2276)
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Customer Q&As
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16 Customer Q&As for Anti-Tyrosine-protein kinase Mer MERTK Antibody Picoband®
Question
We have observed staining in human right adrenal gland cortex. Are there any suggestions? Is anti-MERTK antibody supposed to stain right adrenal gland cortex positively?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
From what I have seen in literature right adrenal gland cortex does express MERTK. From what I have seen in Uniprot.org, MERTK is expressed in right adrenal gland cortex, peripheral blood leukocyte, plasma, among other tissues. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2020-04-07
Question
Would anti-MERTK antibody PA2276 work on primate WB with plasma?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not validated anti-MERTK antibody PA2276 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MERTK antibody PA2276 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
Can you help my question with product PA2276, anti-MERTK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2276 anti-MERTK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
Is this PA2276 anti-MERTK antibody reactive to the isotypes of MERTK?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The immunogen of PA2276 anti-MERTK antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MERTK(132-148aa KDGKELLGAHHAITQFY), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-17
Question
Does PA2276 anti-MERTK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
As indicated on the product datasheet, PA2276 anti-MERTK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-MERTK antibody for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
As indicated on the product datasheet, PA2276 anti-MERTK antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-11
Question
My question regards to test anti-MERTK antibody PA2276 on human plasma for research purposes, then I may be interested in using anti-MERTK antibody PA2276 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
The products we sell, including anti-MERTK antibody PA2276, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-MERTK antibody PA2276. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-10
Question
Our lab were happy with the WB result of your anti-MERTK antibody. However we have been able to see positive staining in right adrenal gland cortex membrane using this antibody. Is that expected? Could you tell me where is MERTK supposed to be expressed?
L. Johnson
Verified customer
Asked: 2018-07-17
Answer
From what I have seen in literature, right adrenal gland cortex does express MERTK. Generally MERTK expresses in membrane. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-07-17
Question
Does anti-MERTK antibody PA2276 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
According to the expression profile of plasma, MERTK is highly expressed in plasma. So, it is likely that anti-MERTK antibody PA2276 will work for WB with plasma.
Boster Scientific Support
Answered: 2018-04-20
Question
I have attached the WB image, lot number and protocol we used for plasma using anti-MERTK antibody PA2276. Please let me know if you require anything else.
G. Anderson
Verified customer
Asked: 2018-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-19
Question
We are currently using anti-MERTK antibody PA2276 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
D. Collins
Verified customer
Asked: 2017-07-27
Answer
The anti-MERTK antibody (PA2276) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-27
Question
I see that the anti-MERTK antibody PA2276 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-26
Question
Is a blocking peptide available for product anti-MERTK antibody (PA2276)?
E. Walker
Verified customer
Asked: 2016-09-15
Answer
We do provide the blocking peptide for product anti-MERTK antibody (PA2276). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-15
Question
My lab would like using your anti-MERTK antibody for phagocytosis studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
R. Krishna
Verified customer
Asked: 2015-10-08
Answer
I appreciate your inquiry. This PA2276 anti-MERTK antibody is validated on hela whole cell lysate, a549 whole cell lysate, nih whole cell lysate, 22rv1 whole cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-08
Question
Do you have a BSA free version of anti-MERTK antibody PA2276 available?
J. Jackson
Verified customer
Asked: 2013-10-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MERTK antibody PA2276 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-10-11
