Anti-NG2 Purified CSPG4 Monoclonal Antibody

Product Name

Anti-NG2 Purified CSPG4 Monoclonal Antibody

View all NG2/MCSP Antibodies

SKU/Catalog Number

M03394-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NG2 Purified CSPG4 Monoclonal Antibody (Catalog# M03394-1). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-NG2 Purified CSPG4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03394-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

7.1

Isotype

Mouse IgG1

Immunogen

Human bone marrow stromal cells infected with SV-40. The mouse monoclonal antibody 7.1 recognizes an extracellular epitope of NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03394-1 is reactive to CSPG4 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

250537 MW

Background of NG2/MCSP

NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03394-1 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CSPG4 (Source: Uniprot.org, NCBI)

Alternative Names

Chondroitin sulfate proteoglycan 4, MSK16, CSPG4, MCSP CSPG4 CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2 chondroitin sulfate proteoglycan 4 chondroitin sulfate proteoglycan 4|chondroitin sulfate proteoglycan 4 (melanoma-associated)|chondroitin sulfate proteoglycan NG2|melanoma chondroitin sulfate proteoglycan|melanoma-associated chondroitin sulfate proteoglycan

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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