Product Info Summary
SKU: | A06867-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NFRKB Antibody Picoband™
SKU/Catalog Number
A06867-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFRKB Antibody Picoband™ catalog # A06867-2. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NFRKB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06867-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFRKB recombinant protein (Position: H270-L994).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06867-2 is reactive to NFRKB in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
175 kDa
Calculated molecular weight
Background of NFRKB
NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06867-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a06867 2 nfrkb primary antibodies wb testing 1 a06867 2 nfrkb primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a06867-2-nfrkb-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of NFRKB using anti-NFRKB antibody (A06867-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFRKB antigen affinity purified polyclonal antibody (Catalog # A06867-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFRKB at approximately 175 kDa. The expected band size for NFRKB is at 139 kDa.
![a06867 2 nfrkb primary antibodies if testing 2 a06867 2 nfrkb primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a06867-2-nfrkb-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of NFRKB using anti-NFRKB antibody (A06867-2).
NFRKB was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NFRKB Antibody (A06867-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a06867 2 nfrkb primary antibodies fcm testing 3 a06867 2 nfrkb primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a06867-2-nfrkb-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of HEL cells using anti-NFRKB antibody (A06867-2).
Overlay histogram showing HEL cells stained with A06867-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFRKB Antibody (A06867-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NFRKB (Source: Uniprot.org, NCBI)
Gene Name
NFRKB
Full Name
Nuclear factor related to kappa-B-binding protein
Weight
Superfamily
NFRKB family
Alternative Names
DKFZp547B2013; DNA-binding protein R kappa-B; INO80 complex subunit G; INO80GDNA-binding protein R kappa B; nuclear factor related to kappaB binding protein; nuclear factor related to kappa-B-binding protein NFRKB INO80G nuclear factor related to kappaB binding protein nuclear factor related to kappa-B-binding protein|DNA-binding protein R kappa B|INO80 complex subunit G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFRKB, check out the NFRKB Infographic
![NFRKB infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFRKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFRKB Antibody Picoband™ (A06867-2)
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