Product Info Summary
SKU: | PB10080 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-NFIA Antibody Picoband®
SKU/Catalog Number
PB10080
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFIA Antibody Picoband® catalog # PB10080. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFIA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10080)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NFIA, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10080 is reactive to NFIA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
55944 MW
Background of NFIA
Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10080 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: JURKAT whole cell, COLO320 whole cell, mouse HEPA1-6 whole cell, mouse SP2/0 whole cell
IHC: mouse liver tissue, rat liver tissue, human mammary cancer tissue
FCM: K562 cell, SiHa cell, U20S cell
Validation Images & Assay Conditions
![pb10080 1 WB anti nfia picoband antibody pb10080 1 WB anti nfia picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb10080-1-WB-anti-nfia-picoband-antibody.jpg)
Click image to see more details
Figure 1. Western blot analysis of NFIA using anti-NFIA antibody (PB10080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates,
Lane 2: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (Catalog # PB10080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIA at approximately 69KD. The expected band size for NFIA is at 56KD.
![pb10080 2 IHC anti nfia picoband antibody pb10080 2 IHC anti nfia picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb10080-2-IHC-anti-nfia-picoband-antibody.jpg)
Click image to see more details
Figure 2. IHC analysis of NFIA using anti-NFIA antibody (PB10080).
NFIA was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIA Antibody (PB10080) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pb10080 3 IHC anti nfia picoband antibody pb10080 3 IHC anti nfia picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb10080-3-IHC-anti-nfia-picoband-antibody.jpg)
Click image to see more details
Figure 3. IHC analysis of NFIA using anti-NFIA antibody (PB10080).
NFIA was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIA Antibody (PB10080) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pb10080 4 IHC anti nfia picoband antibody pb10080 4 IHC anti nfia picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb10080-4-IHC-anti-nfia-picoband-antibody.jpg)
Click image to see more details
Figure 4. IHC analysis of NFIA using anti-NFIA antibody (PB10080).
NFIA was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIA Antibody (PB10080) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pb10080 5 WB anti nfia picoband antibody pb10080 5 WB anti nfia picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb10080-5-WB-anti-nfia-picoband-antibody.jpg)
Click image to see more details
Figure 5. Western blot analysis of NFIA using anti-NFIA antibody (PB10080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 whole cell lysates,
Lane 2: mouse SP2/0 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (Catalog # PB10080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIA at approximately 69KD. The expected band size for NFIA is at 56KD
![pb10080 nfia primary antibodies fc testing 6 pb10080 nfia primary antibodies fc testing 6](https://www.bosterbio.com/media/catalog/product/p/b/pb10080-nfia-primary-antibodies-fc-testing-6.jpg)
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Figure 6. Flow Cytometry analysis of K562 cells using anti-NFIA antibody (PB10080).
Overlay histogram showing K562 cells stained with PB10080 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (PB10080,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![pb10080 nfia primary antibodies fc testing 7 pb10080 nfia primary antibodies fc testing 7](https://www.bosterbio.com/media/catalog/product/p/b/pb10080-nfia-primary-antibodies-fc-testing-7.jpg)
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Figure 7. Flow Cytometry analysis of SiHa cells using anti-NFIA antibody (PB10080).
Overlay histogram showing SiHa cells stained with PB10080 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (PB10080,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![pb10080 nfia primary antibodies fc testing 8 pb10080 nfia primary antibodies fc testing 8](https://www.bosterbio.com/media/catalog/product/p/b/pb10080-nfia-primary-antibodies-fc-testing-8.jpg)
Click image to see more details
Figure 8. Flow Cytometry analysis of U20S cells using anti-NFIA antibody (PB10080).
Overlay histogram showing U20S cells stained with PB10080 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (PB10080,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NFIA (Source: Uniprot.org, NCBI)
Gene Name
NFIA
Full Name
Nuclear factor 1 A-type
Weight
55944 MW
Superfamily
CTF/NF-I family
Alternative Names
Nuclear factor 1 A-type;NF1-A;Nuclear factor 1/A;CCAAT-box-binding transcription factor;CTF;Nuclear factor I/A;NF-I/A;NFI-A;TGGCA-binding protein;NFIA;KIAA1439; NFIA BRMUTD, CTF, NF-I/A, NF1-A, NFI-A, NFI-L nuclear factor I A nuclear factor 1 A-type|CCAAT-box-binding transcription factor|TGGCA-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFIA, check out the NFIA Infographic
![NFIA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFIA Antibody Picoband® (PB10080)
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1 Customer Q&As for Anti-NFIA Antibody Picoband®
Question
We are currently using anti-NFIA antibody PB10080 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
C. Li
Verified customer
Asked: 2017-11-28
Answer
The anti-NFIA antibody (PB10080) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-28