Click image to see more details
Product Info Summary
| SKU: | EK0473-CN |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Canine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Dog Canine Neurotrophin-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK0473-CN
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Dog Canine Neurotrophin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Dog Ntf3 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Dog Canine Neurotrophin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0473-CN)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y139-T257
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0473-CN for more details
Cross-reactivity
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Reactive Species
EK0473-CN is reactive to Ntf3 in Canine samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0473-CN is guaranteed for ELISA in Canine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NT-3
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to Dog Caninea chromosome 12p and Dog Caninea chromosome 6.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0473-CN-CAP | Anti-Canine Ntf3 Pre-coated 96-well strip microplate | 1 |
| EK0473-CN-ST | Canine Ntf3 Standard | 2 vials, 10 ng/tube |
| EK0473-CN-DA | Canine Ntf3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.014 | 0.073 | 0.148 | 0.277 | 0.561 | 0.870 | 1.686 | 2.529 |
Data Example Images
Click image to see more details
Dog Neurotrophin-3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Dog Canine Neurotrophin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 88 | 329 | 682 | 113 | 395 | 753 |
| Standard deviation | 4.84 | 19.74 | 30.01 | 7.12 | 28.44 | 39.16 |
| CV (%) | 5.5 | 6 | 4.4 | 7.2 | 7.2 | 5.2 |
Protein Target Info & Infographic
Gene/Protein Information For Ntf3 (Source: Uniprot.Org, NCBI)
Gene Name
Ntf3
Full Name
Neurotrophin-3
Weight
29588 MW
Superfamily
NGF-beta family
Alternative Names
Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;Ntf3;Ntf-3; NTF3 HDNF, NGF-2, NGF2, NT-3, NT3 neurotrophin 3 neurotrophin-3|nerve growth factor 2|neurotrophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ntf3, check out the Ntf3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Dog Canine Neurotrophin-3 ELISA Kit PicoKine® (EK0473-CN)
Hello CJ!
No publications found for EK0473-CN
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Dog Canine Neurotrophin-3 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Dog Canine Neurotrophin-3 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Dog Canine Neurotrophin-3 ELISA Kit PicoKine®
Question
Q: can you recommend the dilution ratio of serum samples for detection of NT-3 in Dog serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
A. Roberts
Verified customer
Asked: 2020-11-24
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the NT-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-24
Question
Q: if the enzyme conjugated NT-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NT-3 antigen?
Verified Customer
Verified customer
Asked: 2019-06-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-06-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement NT-3 using the EK0473-CN Dog NT-3 Picokine® ELISA Kit?
K. Turner
Verified customer
Asked: 2019-03-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-03-05
Question
Q: Can NT-3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-22
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Perez
Verified customer
Asked: 2017-10-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-10-30
