Click image to see more details
Product Info Summary
| SKU: | MA1070 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IHC, IHC-F, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NF68 Nefl Antibody (Monoclonal, NR4)
SKU/Catalog Number
MA1070
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF68 Nefl Antibody (Monoclonal, NR4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1070)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
NR4
Isotype
Mouse IgG1
Immunogen
Pig spinal cord.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1070 is reactive to Nefl in Human, Mouse, Pig, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
61335 MW
Background of NF-L
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1070 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat, By Heat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat
Positive Control
WB: human SH-SY5Y whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NF68 using anti-NF68 antibody (MA1070).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NF68 antigen affinity purified monoclonal antibody (Catalog # MA1070) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NF68 at approximately 72 kDa. The expected band size for NF68 is at 62 kDa.
Click image to see more details
Figure 2. IHC analysis of NF68 using anti-NF68 antibody (MA1070).
NF68 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NF68 Antibody (MA1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of NF68 using anti-NF68 antibody (MA1070).
NF68 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NF68 Antibody (MA1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nefl (Source: Uniprot.org, NCBI)
Gene Name
Nefl
Full Name
Neurofilament light polypeptide
Weight
61335 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl; NEFL CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110 neurofilament light neurofilament light polypeptide|light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|protein phosphatase 1, regulatory subunit 110
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nefl, check out the Nefl Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nefl: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF68 Nefl Antibody (Monoclonal, NR4) (MA1070)
Hello CJ!
No publications found for MA1070
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NF68 Nefl Antibody (Monoclonal, NR4)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NF68 Nefl Antibody (Monoclonal, NR4)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-NF68 Nefl Antibody (Monoclonal, NR4)
Question
We want to test anti-NF68 antibody (Monoclonal, NR4) MA1070 on rat fetal brain cortex for research purposes, then I may be interested in using anti-NF68 antibody (Monoclonal, NR4) MA1070 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The products we sell, including anti-NF68 antibody (Monoclonal, NR4) MA1070, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-NF68 antibody (Monoclonal, NR4) MA1070 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, pig, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-NF68 antibody (Monoclonal, NR4) (MA1070) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
Can MA1070 be provided carrier free?
Verified customer
Asked: 2019-06-17
Answer
Unfortunately, we can't provide the Anti-NF68 Nefl Antibody (Monoclonal, NR4) (MA1070) in a carrier free format.
Boster Scientific Support
Answered: 2019-06-18
Question
I see that the anti-NF68 antibody (Monoclonal, NR4) MA1070 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-NF68 antibody (Monoclonal, NR4) MA1070. Let me know if you need anything else.
D. Krishna
Verified customer
Asked: 2018-05-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-09
Question
I was wanting to use your anti-NF68 antibody (Monoclonal, NR4) for IHC for rat fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
It shows on the product datasheet, MA1070 anti-NF68 antibody (Monoclonal, NR4) has been tested for IHC, WB on human, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-08
Question
Is a blocking peptide available for product anti-NF68 antibody (Monoclonal, NR4) (MA1070)?
B. Jha
Verified customer
Asked: 2017-08-18
Answer
We do provide the blocking peptide for product anti-NF68 antibody (Monoclonal, NR4) (MA1070). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-18
