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Product Info Summary
| SKU: | A10898 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MRPL44 Antibody
SKU/Catalog Number
A10898
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL44 Antibody (Catalog # A10898). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL44 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10898)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL44.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10898 is reactive to MRPL44 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
37535 MW
Background of MRPL44
Component of the 39S subunit of mitochondrial ribosome. May have a function in the assembly/stability of nascent mitochondrial polypeptides exiting the ribosome.
Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 434:724-731(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10898 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using MRPL44 antibody A10898.
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Western blot analysis of extracts from COS-7 cells, using MRPL44 antibody A10898.
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Immunofluorescence analysis of HuvEc cells, using MRPL44 antibody A10898.
Protein Target Info & Infographic
Gene/Protein Information For MRPL44 (Source: Uniprot.org, NCBI)
Gene Name
MRPL44
Full Name
39S ribosomal protein L44, mitochondrial
Weight
37535 MW
Superfamily
ribonuclease III family
Alternative Names
39S ribosomal protein L44, mitochondrial;L44mt;MRP-L44;3.1.26.-;MRPL44; MRPL44 COXPD16, L44MT, MRP-L44 mitochondrial ribosomal protein L44 39S ribosomal protein L44, mitochondrial|mitochondrial large ribosomal subunit protein mL44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL44, check out the MRPL44 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL44 Antibody (A10898)
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No publications found for A10898
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MRPL44 Antibody
Question
Would anti-MRPL44 antibody A10898 work on goat WB with liver?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Our lab technicians have not tested anti-MRPL44 antibody A10898 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MRPL44 antibody A10898 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-MRPL44 antibody A10898. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
Do you have a BSA free version of anti-MRPL44 antibody A10898 available?
B. Moore
Verified customer
Asked: 2019-09-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MRPL44 antibody A10898 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-MRPL44 antibody A10898 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
The anti-MRPL44 antibody (A10898) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-14
Question
Would anti-MRPL44 antibody A10898 work for WB with liver?
D. Roberts
Verified customer
Asked: 2017-12-25
Answer
According to the expression profile of liver, MRPL44 is highly expressed in liver. So, it is likely that anti-MRPL44 antibody A10898 will work for WB with liver.
Boster Scientific Support
Answered: 2017-12-25
Question
I have a question about product A10898, anti-MRPL44 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Singh
Verified customer
Asked: 2016-04-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10898 anti-MRPL44 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-21
Question
I was wanting to use your anti-MRPL44 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
J. Huang
Verified customer
Asked: 2015-03-10
Answer
As indicated on the product datasheet, A10898 anti-MRPL44 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-10
