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- Table of Contents
Facts about Neurofilament light polypeptide.
Human | |
---|---|
Gene Name: | NEFL |
Uniprot: | P07196 |
Entrez: | 4747 |
Belongs to: |
---|
intermediate filament family |
68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa
Mass (kDA):
61.517 kDA
Human | |
---|---|
Location: | 8p21.2 |
Sequence: | 8; NC_000008.11 (24950955..24956612, complement) |
If you're new to the field of ELISA, you'll find this Boster Bio article particularly helpful. Boster Bio focuses on providing high-affinity primary antibodies and offers optimization guides to remove as many possible sources of error as possible. Boster is a great choice for scientists worldwide and can supply highly specific antibodies for use in different types of experiments. However, if you're not sure whether Boster is the right choice for your research, keep reading to find out more about the NEFL Marker.
If you are looking for a high-affinity antibody against the NEFL marker, you've come to the right place. Boster offers primary antibodies that are highly cited in the research community and validated for use in Western Blotting, Immunohistochemistry, and ELISA. They are backed by technical support and can be used in applications for which high-affinity antibodies are not readily available.
Neurons, also known as neuronal cells, are the major component of the nervous system. They are functionally and morphologically diverse and have cell bodies ranging from 4 to 100 micrometers in diameter. These cell bodies differ in their ability to secrete neurotransmitters. Neuronal markers are diverse, ranging from NeuN, a nuclear antigen, to PGP9.5, a deubiquitinating enzyme. Other neuronal markers include nestin, calretinin, and choline acetyltransferase.
The NEFL marker was first identified in China by a Chinese scientist named Zhou, Tongqing. He then discovered how to manufacture antibodies from antigens. Eventually, he developed a method of stimulating B cells that induce affinity maturation and somatic mutation. This process is the next step in reproducing the full immune response in culture. With these antibodies, researchers will have a powerful tool to detect a variety of infectious agents.
While antibodies are derived from the immune systems of host animals, primary antibodies are unique in that they are made specifically for a specific epitope. The monoclonal antibodies used for research have low non-specific cross-reactivity and minimal lot-to-lot variation. To create polyclonal primary antibodies, scientists immunize host animals and then extract them from their sera or eggs.
Neutralizing antibodies against the NEFL marker are highly effective at blocking viral infection. They recognize and neutralize the virus' outer membrane using a unique peptide motif on the envelope glycoprotein. Neutralizing antibodies are broadly neutralizing and may be helpful in designing vaccines. However, they may also help the virus escape from the body of broadly neutralizing antibodies. The information gathered from neutralizing antibodies will be crucial in designing an effective vaccine.
An ELISA is a versatile, sensitive test that assesses soluble proteins in their native state. This test requires many choices, from sample preparation to antibodies and blocking buffer. The Boster Bio optimization guide answers these questions and more, and offers tips for optimizing your experiments. Below are some frequently asked questions about the ELISA test. To get started, read through the sample preparation section of this guide. There are several key tips for sample preparation included.
Flow cytometry has many variables, but the Boster Bio troubleshooting guides can help you figure out which one is causing your problems and how to correct it. Boster Bio protocols act as primers and quick reference guides that can help you optimize your experiments and obtain better results. Although every researcher encounters some trouble, by following proper protocols and controlling variables, the chances of error are minimized. Moreover, the protocols include optimization and troubleshooting tips to make your experiments run smoothly.
BosterBio's Immunohistochemistry troubleshooting guide provides step-by-step procedures, illustrated workflows, and recommended reagents. An optimized protocol is crucial to achieving high quality staining and the guide outlines the correct procedure for different kinds of preserved tissues. The guide also covers the best sample preparation methods. The guide even covers sample preparation for different types of preserved tissues. The quality of your tissue is a critical step in achieving high-quality staining.
PMID: 3034332 by Julien J.-P., et al. The structure of a human neurofilament gene (NF-L): a unique exon- intron organization in the intermediate filament gene family.
PMID: 12432080 by Perez-Olle R., et al. Effects of Charcot-Marie-Tooth-linked mutations of the neurofilament light subunit on intermediate filament formation.