Product Info Summary
SKU: | M00284 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00284
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody catalog # M00284. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00284)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-κB p65
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00284 is reactive to RELA in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
60219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00284 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Positive Control
IHC: human transitional cell carcinoma of bladder tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of NF-κB p65 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For RELA (Source: Uniprot.org, NCBI)
Gene Name
RELA
Full Name
Transcription factor p65
Weight
60219 MW
Alternative Names
Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;RELA;NFKB3; RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RELA, check out the RELA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RELA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody (M00284)
Hello CJ!
M00284 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Saturated hydrogen alleviates CCl4-induced acute kidney injury via JAK2/STAT3/p65 signaling:
Jiang Peng-Fei,Peng Jun,Tan Han-Yu,Ou-Yang Yun,Qin Gen-Yan,Peng Qing-Hua,Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits,Digital Chinese Medicine,Volume 2,Issue 2,2019,Pages 97-104,ISSN 2589-3777,https://doi.org/10.1016/j.dcmed.2019.09.004.
Species: New Zealand White Rabbit
M00284 usage in article: APP:IHC, SAMPLE:LACRIMAL GLAND CELL, DILUTION: NA
Tan L,Li J,Wang Y,Tan R.Anti-Neuroinflammatory Effect of Alantolactone through the Suppression of the NF-κB and MAPK Signaling Pathways.Cells.2019 Jul 18;8(7):739.doi:10.3390/cells8070739.PMID:31323885;PMCID:PMC6678480.
Species: Mouse,Rat
M00284 usage in article: APP:IF, SAMPLE:BV2 CELL, DILUTION: NA
Xin X,Yao D,Zhang K,Han S,Liu D,Wang H,Liu X,Li G,Huang J,Wang J.Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.Biomed Pharmacother.2019 Jul;115:108870.doi:10.1016/j.biopha.2019.108870.Epub 2019 Apr 23.PMID:31026730.
Species: Mouse
M00284 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:500
Li HN,Yang QQ,Wang WT,Tian X,Feng F,Zhang ST,Xia YT,Wang JX,Zou YW,Wang JY,Zeng XY.Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.J Neuroinflammation.2021 Jul 5;18(1):150.doi:10.1186/s12974-021-02198-9.PMID:34225736;PMCID:PMC8258957.
Species: Rat
M00284 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:1:400
Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
M00284 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION: 1:1000
Wang H,Qiu M,Li Y,Cheng D,Yang D,Yuan W.Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma.Biomed Pharmacother.2020 Jun;126:109866.doi:10.1016/j.biopha.2020.109866.Epub 2020 Feb 27.PMID:32114356.
Species: Mouse
M00284 usage in article: APP:IHC, SAMPLE:MELANOMA TUMOR TISSUE, DILUTION:NA
Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00284 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:2000
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
M00284 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-?B Signaling
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