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Product Info Summary
| SKU: | A08385-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NELF/NSMF Antibody Picoband®
SKU/Catalog Number
A08385-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NELF/NSMF Antibody Picoband® catalog # A08385-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NELF/NSMF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08385-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NELF/NSMF recombinant protein (Position: E14-D527).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08385-1 is reactive to NSMF in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
44,60 kDa
Calculated molecular weight
82457 MW
Background of NSMF
The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08385-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Hacat whole cell, rat liver tissue, rat RH35 whole cell, rat C6 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell, mouse NIH/3T3 whole cell
IHC: human colorectal adenocarcinoma tissue
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NELF/NSMF using anti-NELF/NSMF antibody (A08385-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELF/NSMF antigen affinity purified polyclonal antibody (Catalog # A08385-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELF/NSMF at approximately 44,60 kDa. The expected band size for NELF/NSMF is at 44,60 kDa.
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Figure 2. IHC analysis of NELF/NSMF using anti-NELF/NSMF antibody (A08385-1).
NELF/NSMF was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NELF/NSMF Antibody (A08385-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of Hela cells using anti-NELF/NSMF antibody (A08385-1).
Overlay histogram showing Hela cells stained with A08385-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NELF/NSMF Antibody (A08385-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NSMF (Source: Uniprot.org, NCBI)
Gene Name
NSMF
Full Name
NMDA receptor synaptonuclear signaling and neuronal migration factor
Weight
82457 MW
Superfamily
NSMF family
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 2;ADAM 2;Cancer/testis antigen 15;CT15;Fertilin subunit beta;PH-30;PH30;PH30-beta;ADAM2;FTNB; NSMF HH9, NELF NMDA receptor synaptonuclear signaling and neuronal migration factor NMDA receptor synaptonuclear signaling and neuronal migration factor|nasal embryonic LHRH factor|nasal embryonic luteinizing hormone-releasing hormone factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NSMF, check out the NSMF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NSMF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NELF/NSMF Antibody Picoband® (A08385-1)
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