Product Info Summary
SKU: | A08385 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NELF NSMF Antibody
SKU/Catalog Number
A08385
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NELF NSMF Antibody (Catalog # A08385). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NELF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NELF NSMF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08385)
Host
Rabbit
Contents
NELF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF. The immunogen is located within amino acids 230 - 280 of NELF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NINJ1 antibody is human, mouse and rat reactive.
Reactive Species
A08385 is reactive to NELF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
60143 MW
Background of NSMF
NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08385 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μg/ml.
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Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 μg/ml.
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Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 μg/mL.
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Immunofluorescence of NELF in mouse brain tissue with NELF Antibody at 20 μg/mL.
Green: NELF antibody (A08385)
Red: Phylloidin staining
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For NELF (Source: Uniprot.org, NCBI)
Gene Name
NELF
Full Name
NMDA receptor synaptonuclear signaling and neuronal migration factor
Weight
60143 MW
Superfamily
NSMF family
Alternative Names
HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor NSMF HH9, NELF NMDA receptor synaptonuclear signaling and neuronal migration factor NMDA receptor synaptonuclear signaling and neuronal migration factor|nasal embryonic LHRH factor|nasal embryonic luteinizing hormone-releasing hormone factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NELF, check out the NELF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NELF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NELF NSMF Antibody (A08385)
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