Product Info Summary
SKU: | A02482-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-NEFL/NF-L Antibody Picoband®
SKU/Catalog Number
A02482-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NEFL/NF-L Antibody Picoband® catalog # A02482-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEFL/NF-L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02482-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human NEFL/NF-L recombinant protein (Position: F4-A463).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02482-1 is reactive to NEFL in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
22105 MW
Background of NF-L
Neurofilament light polypeptide (NFL), also known as neurofilament light chain, is a neurofilament protein that in humans is encoded by the NEFL gene. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02482-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human SH-SY5Y whole cell, rat brain tissue, mouse brain tissue
IHC: mouse spinal cord tissue, rat spinal cord tissue, mouse spinal cord tissue
IF: mouse brain tissue, rat brain tissue
FCM: 293T cell
Validation Images & Assay Conditions
![a02482 1 nefl primary antibodies wb testing 1_1 a02482 1 nefl primary antibodies wb testing 1_1](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-wb-testing-1_1.jpg)
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Figure 1. Western blot analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEFL/NF-L antigen affinity purified polyclonal antibody (Catalog # A02482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEFL/NF-L at approximately 72KD. The expected band size for NEFL/NF-L is at 72KD.
![a02482 1 nefl primary antibodies ihc testing 2 a02482 1 nefl primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
NEFL/NF-L was detected in paraffin-embedded section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-NEFL/NF-L Antibody (A02482-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a02482 1 nefl primary antibodies fcm testing 3 a02482 1 nefl primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of 293T cells using anti-NEFL/NF-L antibody (A02482-1).
Overlay histogram showing 293T cells stained with A02482-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEFL/NF-L Antibody (A02482-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a02482 1 nefl primary antibodies ihc testing 4 a02482 1 nefl primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-ihc-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
NEFL/NF-L was detected in paraffin-embedded section of rat spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-NEFL/NF-L Antibody (A02482-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a02482 1 nefl primary antibodies ihc testing 5 a02482 1 nefl primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-ihc-testing-5.jpg)
Click image to see more details
Figure 5. IHC analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
NEFL/NF-L was detected in paraffin-embedded section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-NEFL/NF-L Antibody (A02482-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a02482 1 nefl primary antibodies if testing 6 a02482 1 nefl primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
NEFL/NF-L was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NEFL/NF-L Antibody (A02482-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a02482 1 nefl primary antibodies if testing 7 a02482 1 nefl primary antibodies if testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a02482-1-nefl-primary-antibodies-if-testing-7.jpg)
Click image to see more details
Figure 7. IF analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1).
NEFL/NF-L was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NEFL/NF-L Antibody (A02482-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For NEFL (Source: Uniprot.org, NCBI)
Gene Name
NEFL
Full Name
Neurofilament light polypeptide
Weight
22105 MW
Superfamily
intermediate filament family
Alternative Names
68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa NEFL CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110 neurofilament light neurofilament light polypeptide|light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|protein phosphatase 1, regulatory subunit 110
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFL, check out the NEFL Infographic
![NEFL infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEFL/NF-L Antibody Picoband® (A02482-1)
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Customer Q&As
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1 Customer Q&As for Anti-NEFL/NF-L Antibody Picoband®
Question
can you tell me the sequence and UNIPROT number?
Verified customer
Asked: 2022-11-29
Answer
The immunogen sequence for A02482-1 is F4-A463 (UNIPROT number: P07196). This info can be found on the product page. https://www.bosterbio.com/anti-nefl-nf-l-picoband-trade-antibody-a02482-1-boster.html
Boster Scientific Support
Answered: 2022-11-29