Anti-NEFH Antibody (Monoclonal, N52)

Product Name

Anti-NEFH Antibody (Monoclonal, N52)

View all NF-H Antibodies

SKU/Catalog Number

MA1071

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEFH Antibody (Monoclonal, N52) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

N52

Isotype

Mouse IgG1

Immunogen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1071 is reactive to NEFH in Human, Mouse, Rat

Applications

MA1071 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

115378 MW

Background of NF-H

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5ml, Human, mouse, rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of NEFH using anti-NEFH antibody (MA1071).
NEFH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Click image to see more details

Figure 2. Western blot analysis of NEFH using anti-NEFH antibody (MA1071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NEFH antigen affinity purified monoclonal antibody (Catalog # MA1071)) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NEFH at approximately 200KD. The expected band size for NEFH is at 112KD.

Click image to see more details

Figure 3. IHC analysis of NEFH using anti-NEFH antibody (MA1071).
NEFH was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For NEFH (Source: Uniprot.org, NCBI)

Alternative Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H NEFH CMT2CC, NFH neurofilament heavy neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa

For more info on NEFH, check out the NEFH Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NEFH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the NEFH Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis