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Product Info Summary
| SKU: | MA1071 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NEFH Antibody (Monoclonal, N52)
SKU/Catalog Number
MA1071
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEFH Antibody (Monoclonal, N52) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
N52
Isotype
Mouse IgG1
Immunogen
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1071 is reactive to Nefh in Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
200 kDa
Calculated molecular weight
115378 MW
Background of NF-H
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1071 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5ml, Human, mouse, rat
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: rat brain tissue, human glioma tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of NEFH using anti-NEFH antibody (MA1071).
NEFH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of NEFH using anti-NEFH antibody (MA1071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NEFH antigen affinity purified monoclonal antibody (Catalog # MA1071)) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NEFH at approximately 200KD. The expected band size for NEFH is at 112KD.
Click image to see more details
Figure 3. IHC analysis of NEFH using anti-NEFH antibody (MA1071).
NEFH was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nefh (Source: Uniprot.org, NCBI)
Gene Name
Nefh
Full Name
Neurofilament heavy polypeptide
Weight
115378 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh; NEFH CMT2CC, NFH neurofilament heavy neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nefh, check out the Nefh Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nefh: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEFH Antibody (Monoclonal, N52) (MA1071)
Hello CJ!
MA1071 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
MA1071 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats
MicroRNA-30b regulates expression of the sodium channel Nav17 in nerve injury-induced neuropathic pain in the rat
Neuron-derived IgG Protects Neurons from Complement-dependent Cytotoxicity
An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots
Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice
Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia
Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Huang H, Lin F, Jiang J, Chen Y, Mei A, Zhu P. Stem Cell Res Ther. 2017 Oct 10;8(1):223. doi: 10.1186/s13287-017-0671-6. Effects of intra-arterial transplantation of adipose-derived stem cells on the expression of netrin-1 and its receptor DCC in ...
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Customer Q&As
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5 Customer Q&As for Anti-NEFH Antibody (Monoclonal, N52)
Question
How does your MA1071 NEFH antibody differ from SIGMA (ex-Millipore) Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat. no. MAB5262 clone: RT97? We intend to use it for immunohistochemistry on Fromalin Fixed tissues and paraffin embedded tissues.
Verified customer
Asked: 2019-11-06
Answer
The main difference would be the immunogen. Anti-NEFH antibody (Monoclonal, N52) MA1071 was raised against C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. (uniprot: P16884). While the Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat.no. MAB5262 clone: RT97 Immunogen is Rat brain protein preparation (uniprot: P12036). Our MA1071 antibody should work for mouse samples with IHC-P and IHC-F.
Boster Scientific Support
Answered: 2019-11-06
Question
Is MA1071 suitable for immunostaining of mouse long bone nerve fibers?
Verified customer
Asked: 2019-10-06
Answer
Yes, the Anti-NEFH Antibody (Monoclonal, N52) (MA1071) is suitable for the immunostaining of mouse long bone nerve fibers.
Boster Scientific Support
Answered: 2019-10-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NEFH antibody (Monoclonal, N52) MA1071. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-NEFH antibody (Monoclonal, N52) MA1071 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
P. Carter
Verified customer
Asked: 2018-01-19
Answer
The anti-NEFH antibody (Monoclonal, N52) (MA1071) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-19
Question
Does anti-NEFH antibody (Monoclonal, N52) MA1071 work on zebrafish IHC with brain?
Z. Li
Verified customer
Asked: 2017-08-31
Answer
Our lab technicians have not tested anti-NEFH antibody (Monoclonal, N52) MA1071 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-NEFH antibody (Monoclonal, N52) MA1071 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-31
