Anti-NEDD4L (phospho-S448) Antibody

Product Name

Anti-NEDD4L (phospho-S448) Antibody

View all NEDD4L Antibodies

SKU/Catalog Number

A01595S448

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NEDD4L (phospho-S448) Antibody catalog # A01595S448. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEDD4L (phospho-S448) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01595S448)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NR4A2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01595S448 is reactive to NEDD4L in Human, Mouse, Rat

Applications

A01595S448 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

111932 MW

Background of NEDD4L

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Ouyang P., J. Cell Biol. 135:1027-1042(1996).
Degen W.G.J., Biochim. Biophys. Acta 1444-384-394(1999).
Shi Y., Oncogene 19:289-297(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-NEDD4L (phospho-S448) Antibody A01595S448

Western blot analysis of extracts of various cell lines

Gene/Protein Information For NEDD4L (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase NEDD4-like; EC 6.3.2; EC 6.3.2.-; FLJ33870; hNedd4-2; KIAA0439ubiquitin-protein ligase NEDD4-like; NEDD4.2; NEDD4-2; NEDL3; neural precursor cell expressed, developmentally down-regulated 4-like; neural precursor cell expressed, developmentally down-regulated gene 4-like; RSP5ubiquitin-protein ligase Rsp5 NEDD4L NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2 NEDD4 like E3 ubiquitin protein ligase E3 ubiquitin-protein ligase NEDD4-like|HECT-type E3 ubiquitin transferase NED4L|neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase|ubiquitin-protein ligase Rsp5

For more info on NEDD4L, check out the NEDD4L Infographic

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In this infographic, you will see the following information for NEDD4L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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