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Product Info Summary
| SKU: | A01413-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®
View all epithelial Sodium Channel alpha Antibodies
SKU/Catalog Number
A01413-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® catalog # A01413-1. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01413-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SCNN1A, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat SCNN1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01413-1 is reactive to SCNN1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated molecular weight
75.704kDa
Background of epithelial Sodium Channel alpha
The SCNN1A gene encodes the alpha subunit of the epithelial sodium channel (ENaC), a constitutively active channel that allows the flow of sodium ions from the lumen into epithelial cells across the apical cell membrane. The ENaC channel, which is regulated by the renin-angiotensin-aldosterone system, has a central role in the regulation of extracellular fluid volume and blood pressure. The other subunits are encoded by the beta (SCNN1B), gamma (SCNN1G), and delta (SCNN1D) genes. This SCNN1A gene is mapped to 12p13.31. Mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01413-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/ Immunofluorescence, 2μg/ml
Positive Control
WB: human COLO-320 whole cell, human HepG2 whole cell, human A549 whole cell
ICC/IF: A431 cell, A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SCNN1A using anti-SCNN1A antibody (A01413-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCNN1A antigen affinity purified polyclonal antibody (Catalog # A01413-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCNN1A at approximately 76KD. The expected band size for SCNN1A is at 76KD.
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Figure 2. IF analysis of SCNN1A using anti-SCNN1A antibody (A01413-1).
SCNN1A was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SCNN1A Antibody (A01413-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. IF analysis of SCNN1A using anti-SCNN1A antibody (A01413-1).
SCNN1A was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SCNN1A Antibody (A01413-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SCNN1A (Source: Uniprot.org, NCBI)
Gene Name
SCNN1A
Full Name
Amiloride-sensitive sodium channel subunit alpha
Weight
75.704kDa
Superfamily
amiloride-sensitive sodium channel (TC 1.A.6) family
Alternative Names
Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na (+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1A; SCNN1 SCNN1A BESC2, ENaCa, ENaCalpha, LIDLS3, SCNEA, SCNN1 sodium channel epithelial 1 subunit alpha amiloride-sensitive sodium channel subunit alpha|alpha ENaC-2|alpha-ENaC|alpha-NaCH|amiloride-sensitive epithelial sodium channel alpha subunit|amiloride-sensitive sodium channel subunit alpha 2|epithelial Na(+) channel subunit alpha|nasal epithelial sodium channel alpha subunit|nonvoltage-gated sodium channel 1 subunit alpha|sodium channel epithelial 1 alpha subunit|sodium channel, non voltage gated 1 alpha subunit|sodium channel, non-voltage-gated 1 alpha|sodium channel, nonvoltage-gated 1 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCNN1A, check out the SCNN1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCNN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® (A01413-1)
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1 Customer Q&As for Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®
Question
We are currently using anti-epithelial Sodium Channel alpha/SCNN1A antibody A01413-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-epithelial Sodium Channel alpha/SCNN1A antibody (A01413-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
