Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody

Product Name

Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody

View all NEDD4L Antibodies

SKU/Catalog Number

M01595

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody catalog # M01595. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01595)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GOA-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NEDD4-2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01595 is reactive to NEDD4L in Human, Mouse, Rat

Applications

M01595 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

111932 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NEDD4L using anti-NEDD4L antibody (M01595).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4L antigen affinity purified monoclonal antibody (Catalog # M01595) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4L at approximately 130 kDa. The expected band size for NEDD4L is at 112 kDa.

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Western blot analysis of NEDD4-2 expression in mouse spleen tissue.

Gene/Protein Information For NEDD4L (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase NEDD4-like; EC 6.3.2; EC 6.3.2.-; FLJ33870; hNedd4-2; KIAA0439ubiquitin-protein ligase NEDD4-like; NEDD4.2; NEDD4-2; NEDL3; neural precursor cell expressed, developmentally down-regulated 4-like; neural precursor cell expressed, developmentally down-regulated gene 4-like; RSP5ubiquitin-protein ligase Rsp5 NEDD4L NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2 NEDD4 like E3 ubiquitin protein ligase E3 ubiquitin-protein ligase NEDD4-like|HECT-type E3 ubiquitin transferase NED4L|neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase|ubiquitin-protein ligase Rsp5

For more info on NEDD4L, check out the NEDD4L Infographic

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In this infographic, you will see the following information for NEDD4L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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