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Product Info Summary
| SKU: | M01595 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody
SKU/Catalog Number
M01595
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody catalog # M01595. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01595)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOA-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NEDD4-2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01595 is reactive to NEDD4L in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130 kDa
Calculated molecular weight
111932 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01595 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IP 1:50
Positive Control
WB: human K562 whole cell, human Hela whole cell, human RT4 whole cell, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NEDD4L using anti-NEDD4L antibody (M01595).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4L antigen affinity purified monoclonal antibody (Catalog # M01595) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4L at approximately 130 kDa. The expected band size for NEDD4L is at 112 kDa.
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Western blot analysis of NEDD4-2 expression in mouse spleen tissue.
Protein Target Info & Infographic
Gene/Protein Information For NEDD4L (Source: Uniprot.org, NCBI)
Gene Name
NEDD4L
Full Name
E3 ubiquitin-protein ligase NEDD4-like
Weight
111932 MW
Alternative Names
E3 ubiquitin-protein ligase NEDD4-like;6.3.2.-;NEDD4.2;Nedd4-2;NEDD4L;KIAA0439, NEDL3; NEDD4L NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2 NEDD4 like E3 ubiquitin protein ligase E3 ubiquitin-protein ligase NEDD4-like|HECT-type E3 ubiquitin transferase NED4L|neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase|ubiquitin-protein ligase Rsp5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEDD4L, check out the NEDD4L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEDD4L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody (M01595)
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