Anti-TMP21 TMED10 Antibody

Product Name

Anti-TMP21 TMED10 Antibody

View all Tmp21/p23 Antibodies

SKU/Catalog Number

A07234

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TMP21 TMED10 Antibody (Catalog # A07234). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TMP21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TMP21 TMED10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07234)

Host

Rabbit

Contents

TMP21 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TMP21 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TMP21. The immunogen is located within amino acids 160 - 210 of TMP21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TTYH1 antibody is human specific. Multiple isoforms of TTYH1 are known to exist.

Reactive Species

A07234 is reactive to TMED10 in Human, Mouse, Rat

Applications

A07234 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

24976 MW

Background of Tmp21/p23

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TMP21 in Raji cell lysate with TMP21 antibody at (A) 0.5 and (B) 1 and (C) 2 μg/mL.

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Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 2.5 μg/mL.

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Immunofluorescence of TMP21 in Human Brain cells with TMP21 antibody at 20 μg/mL.

Gene/Protein Information For TMED10 (Source: Uniprot.org, NCBI)

Alternative Names

21 kDa transmembrane-trafficking protein; p23; P24(DELTA); p24delta; S31I125; S31III125; TMED10; Tmp21; TMP21Tmp-21-I; transmembrane emp24 domain-containing protein 10; transmembrane emp24-like trafficking protein 10 (yeast); Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein TMED10 P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, p23, p24d1 transmembrane p24 trafficking protein 10 transmembrane emp24 domain-containing protein 10|21 kDa transmembrane trafficking protein|p24 family protein delta-1|p24delta|p24delta1|testicular tissue protein Li 206|transmembrane emp24-like trafficking protein 10|transmembrane protein Tmp21

For more info on TMED10, check out the TMED10 Infographic

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In this infographic, you will see the following information for TMED10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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