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Product Info Summary
| SKU: | A01327-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NDRG1 Antibody Picoband®
SKU/Catalog Number
A01327-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NDRG1 Antibody Picoband® catalog # A01327-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDRG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01327-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDRG1 recombinant protein (Position: S2-N220).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01327-1 is reactive to NDRG1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
60066 MW
Background of NDRG1
Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01327-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human U-87MG whole cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NDRG1 using anti-NDRG1 antibody (A01327-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified polyclonal antibody (Catalog # A01327-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 46KD. The expected band size for NDRG1 is at 46KD.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-NDRG1 antibody (A01327-1).
Overlay histogram showing PC-3 cells stained with A01327-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDRG1 Antibody (A01327-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NDRG1 (Source: Uniprot.org, NCBI)
Gene Name
NDRG1
Full Name
Protein NDRG1
Weight
60066 MW
Superfamily
NDRG family
Alternative Names
Protein NDRG1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42; NDRG1; CAP43; DRG1; RTP NDRG1 CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5 N-myc downstream regulated 1 protein NDRG1|N-myc downstream-regulated gene 1 protein|differentiation-related gene 1 protein|nickel-specific induction protein Cap43|protein regulated by oxygen-1|reducing agents and tunicamycin-responsive protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDRG1, check out the NDRG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDRG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDRG1 Antibody Picoband® (A01327-1)
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