Anti-NDFIP1 Antibody Picoband™

Product Name

Anti-NDFIP1 Antibody Picoband™

View all Ndfip1 Antibodies

SKU/Catalog Number

A04644-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NDFIP1 Antibody Picoband™ catalog # A04644-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NDFIP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04644-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NDFIP1 recombinant protein (Position: A10-Y221).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04644-2 is reactive to Ndfip1 in Human, Mouse, Rat

Applications

A04644-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

Background of Ndfip1

Nedd4 family interacting protein 1 is a protein that in humans is encoded by the NDFIP1 gene. The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NDFIP1 using anti-NDFIP1 antibody (A04644-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse lung tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDFIP1 antigen affinity purified polyclonal antibody (Catalog # A04644-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDFIP1 at approximately 28 kDa. The expected band size for NDFIP1 is at 25 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-NDFIP1 antibody (A04644-2).
Overlay histogram showing RT4 cells stained with A04644-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDFIP1 Antibody (A04644-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Ndfip1 (Source: Uniprot.org, NCBI)

Alternative Names

Breast cancer-associated protein SGA-1M; MGC10924; N4WBP5; N4WBP5Putative NF-kappa-B-activating protein 164; NDFIP1; Nedd4 family interacting protein 1; NEDD4 family-interacting protein 1; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NFKB and MAPK-activating protein Ndfip1|0610010M22Rik, N4wbp5|Nedd4 family interacting protein 1|NEDD4 family-interacting protein 1|NEDD4 WW domain-binding protein 5|Nedd4 WW binding protein 5

For more info on Ndfip1, check out the Ndfip1 Infographic

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In this infographic, you will see the following information for Ndfip1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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