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Product Info Summary
| SKU: | P04629 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody
View all KCC2/SLC12A5 Antibodies
SKU/Catalog Number
P04629
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody (Catalog # P04629). Tested in WB, IP applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04629)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P04629 is reactive to KCC2 in Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
126184 MW
Background of KCC2/SLC12A5
KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser-940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04629 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IP: 40ul of antibody per 1mg lysate
Validation Images & Assay Conditions
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For KCC2 (Source: Uniprot.org, NCBI)
Gene Name
KCC2
Full Name
Solute carrier family 12 member 5
Weight
126184 MW
Superfamily
SLC12A transporter family
Alternative Names
Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody Slc12a5|Kcc2|solute carrier family 12 member 5|solute carrier family 12 member 5|K-Cl cotransporter 2|electroneutral potassium-chloride cotransporter 2|furosemide-sensitive K-Cl cotransporter|neuronal K-Cl cotransporter|neuronal-specific K-Cl cotransporter|solute carrier family 12 (potassium-chloride transporter), member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCC2, check out the KCC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody (P04629)
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No publications found for P04629
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody
Question
Would P04629 anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
You can see on the product datasheet, P04629 anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-13
Question
I see that the anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody P04629 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-15
Question
Can you help my question with product P04629, anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Anderson
Verified customer
Asked: 2019-08-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04629 anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody P04629. Let me know if you need anything else.
M. Taylor
Verified customer
Asked: 2018-05-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-11
Question
Here is the WB image, lot number and protocol we used for caudate nucleus using anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) antibody P04629. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-26
